FIG 6.

Treatment with sulforaphane (SFN) decreases Mycobacterium growth by upregulating the expression of Nramp1 and HO-1. (A) Mycobacterial outgrowths in the lung, liver, and spleen of wild-type mice 1 month after intranasal inoculation of 1 × 10⁷ CFU of MAC with or without treatment with sulforaphane. The results are expressed as CFU per organ. (B) Expression of Nramp1 and HO-1 in the lungs of wild-type mice 1 month after intranasal inoculation of 1 × 10⁷ CFU of MAC (solid bars). Control mice were administered saline intranasally and the same amount of dimethyl sulfoxide (DMSO) with PBS intraperitoneally (open bars). Experiments were performed in duplicate with four mice in each group. Differences between SFN-treated Nrf2^−/− mice and control Nrf2^−/− mice after MAC infection were significant (*, P < 0.05). Data are means and SEM.