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[Preprint]. 2021 Jul 9:2021.02.02.429458. Originally published 2021 Feb 10. [Version 2] doi: 10.1101/2021.02.02.429458

Fig. 2. Binding properties and specificity of isolated naive antibodies.

Fig. 2.

(A) ELISA binding heat map of 44 naive IgGs. Binding to wildtype SARS-CoV-2 RBD (SARS-2), ΔRBM, individual RBD glycan variants, circulating variants, related CoVs, hCoVs and polyreactivity antigens. (B) Pearson correlation analysis of SARS-CoV-2 RBD affinities and HCDR3 length. (C) ELISA EC50s for IgGs with detectable SARS-CoV-2 RBD binding (n = 36) based on kappa or lambda gene usage. Red bars indicate the mean EC50 values. (D) Wildtype SARS-CoV-2 RBD ELISA EC50s plotted against EC50s for B.1.1.7 RBD (E) B.1.351 RBD. (F) Proportion of SARS-CoV-2 RBD binders with detectable ELISA affinity for variants of concern (VOC) B.1.1.7 and B.1.351 RBDs. (G) ELISA EC50 values to related sarbecovirus RBDs displayed in decreasing order of paired-sequence identity.