. 2021 Jan 18;32(4):296–305. doi: 10.1097/WNR.0000000000001596

Table 2.

Effect of fisetin on SCI- induced alteration in mRNA expressions of TNF-α, IL-1β, IL-6, iNOs, COX-II, BDNF, Bax, Bcl-2 and caspase-3 in the spinal cord of a rat

Parameter	Normal	Sham	SCI control	Methylprednisolone (30)	Fisetin (10)	Fisetin (20)	Fisetin (40)
TNF-α/β-actin ratio	0.15 ± 0.03	0.33 ± 0.03	1.46 ± 0.02^#,&	0.38 ± 0.06*^,$	1.37 ± 0.03	0.93 ± 0.02*^,$	0.69 ± 0.05*^,$
IL-1β/β-actin ratio	0.51 ± 0.07	0.76 ± 0.08	2.16 ± 0.04^#,&	0.78 ± 0.06*^,$	2.09 ± 0.08	1.56 ± 0.08*^,$	1.02 ± 0.07*^,$
IL-6/β-actin ratio	0.67 ± 0.04	0.80 ± 0.03	1.49 ± 0.05^#,&	0.86 ± 0.06*^,$	1.37 ± 0.02	1.02 ± 0.05*^,$	0.92 ± 0.04*^,$
iNOs/β-actin ratio	0.44 ± 0.05	0.73 ± 0.09	2.07 ± 0.07^#,&	0.78 ± 0.06*^,$	1.92 ± 0.08	1.39 ± 0.07*^,$	0.94 ± 0.02*^,$
COX-II/β-actin ratio	0.15 ± 0.03	0.28 ± 0.02	1.06 ± 0.03^#,&	0.52 ± 0.05*^,$	0.96 ± 0.04	0.75 ± 0.05*^,$	0.54 ± 0.02*^,$
BDNF/β-actin ratio	1.03 ± 0.09	1.03 ± 0.02	0.28 ± 0.04^#,&	0.98 ± 0.06*^,$	0.44 ± 0.06	0.71 ± 0.05*^,$	0.91 ± 0.06*^,$
Bax/β-actin ratio	0.39 ± 0.06	0.45 ± 0.05	1.47 ± 0.07^#,&	0.61 ± 0.06*^,$	1.35 ± 0.05	1.11 ± 0.07*^,$	0.80 ± 0.05*^,$
Bcl-2/β-actin ratio	3.41 ± 0.07	3.08 ± 0.04	0.87 ± 0.09^#,&	2.94 ± 0.10*^,$	1.36 ± 0.13	1.87 ± 0.16*^,$	2.79 ± 0.08*^,$
Caspase-3/β-actin ratio	0.36 ± 0.03	0.41 ± 0.03	1.21 ± 0.02^#,&	0.60 ± 0.06*^,$	1.06 ± 0.04	0.92 ± 0.05*^,$	0.60 ± 0.03*^,$

Data are expressed as mean ± SEM (n = 4) and one-way ANOVA followed by Tukey’s multiple range test.

ANOVA, analysis of variance; COX-II, cyclooxygenase-II; Bax, Bcl-2-associated X; Bcl-2, B-cell lymphoma 2; BDNF, brain-derived neurotrophic factor; iNOS, inducible nitric oxide synthase; IL-1β, interleukin-1 beta; IL-6, interleukin-6; SCI, spinal cord injury; TNF-α, tumor necrosis factor-alpha.

^#P < 0.05 as compared with sham group.

^&P < 0.05 as compared with normal.

*P < 0.05 as compared with the SCI control group.

^$P < 0.05 as compared with one another.