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. 2021 Jan 8;40(7):1255–1268. doi: 10.1038/s41388-020-01595-3

Fig. 4. Dysregulated genes following co-transfection of miR-509-3p and miR-509-3-5p and target prediction.

Fig. 4

A Volcano plot of dysregulated genes generated via mRNA sequencing of transfected cells. Genes expressed at <10 FPKM both in both negative control-transfected and co-transfected ES-2 cells were excluded. B Functional annotation of the dysregulated genes by following co-transfection in terms of KEGG pathways. C Number of putative targets of miR-509-3p or miR-509-3-5p. The online programs TargetScanHuman7.2, DIANA-microT-CDS, miRWalk3.0, and miRanda were used. D Venn diagrams for dysregulated genes and putative targets of the miRNAs. E Predicted interaction between YAP1 and miR-509-3p. F Validation of YAP1 suppression following co-transfection of miR-509-3p and miR-509-3-5p at the transcriptional and protein levels. Relative expression of YAP1/GAPDH was compared using Student’s t test (**p < 0.01).