PCSK9 mediates the oxidative low-density lipoprotein-induced pyroptosis of vascular endothelial cells via the UQCRC1/ROS pathway

Abstract

The present study aimed to explore the role and mechanisms of proprotein convertase subtilisin/kexin type 9 (PCSK9) in the oxidized low-density lipoprotein (oxLDL)-induced pyroptosis of vascular endothelial cells. For this purpose, human umbilical vein endothelial cells (HUVECs) were incubated with oxLDL (100 µg/ml) for 24 h to induce pyroptosis, which was detected using PI/hoechst33342 double staining. The expression of pyroptosis-associated molecules was measured by western blot analysis and RT-qPCR. Reactive oxygen species (ROS) and membrane potential were examined through ROS probe and JC-1 staining, respectively. PCSK9 and mitochondrial ubiquinol-cytochrome c reductase core protein 1 (UQCRC1) protein were knocked down by small interfering RNA (siRNA). PCSK9 was overexpressed by lentivirus. The results revealed that oxLDL induced HUVEC injury, pyroptosis and inflammatory factor release, and upregulated the expression of PCSK9 protein in the HUVECs in a concentration-dependent manner. The silencing of PCSK9 expression with siRNA suppressed the oxLDL-induced damage to HUVECs, the release of inflammatory substances and the occurrence of pyroptosis. In addition, oxLDL inhibited UQCRC1 expression, promoted mitochondrial membrane potential collapse and damaged mitochondrial function; however, these processes were reversed by the silencing of PCSK9. PCSK9 overexpression induced the pyroptosis of HUVECs, the generation of ROS and the disorder of mitochondrial function by inhibiting UQCRC1. Therefore, PCSK9 mediates the oxLDL-induced pyroptosis of vascular endothelial cells via the UQCRC1/ROS pathway.

Introduction

Atherosclerosis (AS) is a chronic inflammatory arterial angiopathy and a common cause of cardiovascular diseases (1). The common pathological changes of AS include endothelial cell dysfunction, lipid deposition and vascular wall thickening (2). Vascular endothelial cells constitute the inner lining of arterial vessels, and their morphological integrity and functional homeostasis play an important role. Damaged integrity and dysfunction in vascular endothelial cells can initiate AS and thus lead to the development of severe clinical acute events, such as myocardial infarction and stroke (3). Proprotein convertase subtilisin/kexin type 9 (PCSK9) is an important risk factor of AS that induces disordered lipid metabolism by cutting low-density lipoprotein receptor (4), and participates in the emergence and progression of AS through the non-lipid metabolic pathway, resulting in vascular endothelial cell death and vascular inflammation (5). However, the underlying mechanisms remain unclear.

Programmed cell death, such as autophagy, apoptosis and pyroptosis, occurs in pathological and disease progression. Cell pyroptosis is accompanied by the release of inflammatory mediators, such as interleukin (IL)-1β and IL-18 (6,7), which can induce cell dysfunction and plaque formation (8). Oxidized low-density lipoprotein (oxLDL), a risk factor for the development of AS, can cause mitochondrial dysfunction and increase reactive oxygen species (ROS) levels in human umbilical vein endothelial cells (HUVECs), thereby inducing the pyroptosis of endothelial cells and the release of inflammatory mediators (9). However, the involvement of PCSK9 in the pyroptosis of vascular endothelial cells remains unclear.

As the energy supplying organelle, the mitochondria regulate cell functions and adjust homeostasis by releasing related signal molecules (10). Homeostasis disruption causes mitochondrial dysfunction, which reduces mitochondrial membrane potential, decreases ATP level, and increases ROS production, leading to cell dysfunction. oxLDL can damage mitochondrial function, induce ROS release and cause endothelial cell pyroptosis (9). ROS are important mediators in the signaling pathway of pyroptosis. Abnormal shear stress can promote PCSK9 expression in endothelial cells (11), and PCSK9 upregulation increases ROS levels (12). Therefore, PCSK9 possibly causes pyroptosis. The present study aimed to determine whether PCSK9 regulates the pyroptosis of vascular endothelial cells through the ROS pathway.

Materials and methods

Cell lines and cell culture

HUVECs (Science Cell Research Laboratories) were cultured in endothelial cell medium (Science Cell Research Laboratories) at 37°C with 5% CO₂. The small-interfering RNA (siRNA) kit was purchased from Guangzhou RiboBio Co., Ltd. In brief, HUVECs at a density of 30-50% were transfected with siRNA (RiboBio Transfection reagent, 50 µM) (siPCSK9, 3′-GAG GTG TAT CTC CTA GAC A-5′; siUQCRC1, 3′-GTG CAC ACT TTG TCT GTG A-5′) or lentivirus (LV)-PCSK9 (Shanghai GeneChem Co., Ltd.; with Genechem HitransG, MOI=50) in the presence or absence of PCSK9 and then incubated with oxLDL (100 µg/ml; Yiyuan Biotechnology Co., Ltd.) for 24 h in an incubator.

Detection of cell pyroptosis

Lactate dehydrogenase (LDH) release was measured with an LDH kit (Jinagcheng bioengineering institute, Nanjing, China) and Hoechst 33342/propidium iodide (PI) staining were used to evaluate pyroptosis. For LDH release detection, 25 µl of cell supernatant and 25 µl of substrate were incubated at 37°C for 15 min. Subsequently, 2,4-dinitrophenylhydrazine (25 µl) and 0.4 mol/l NaOH solution (250 µl) (Nanjing Jinagcheng Bioengineering Institute) were added. Following incubation at room temperature for 5 min, the absorbance was determined using a spectrophotometric microplate reader (iMark-03; Bio-Rad Laboratories, Inc.).

For Hoechst 33342/PI staining, the HUVECs were first washed thrice with phosphate-buffered saline (PBS), followed by the addition of 10 µl of Hoechst 33342 solution, and incubation at 37°C in the dark for 10 min. After removing the dye, the cells were washed times with PBS, added with 5 µl of PI dye, and then incubated at 37°C in the dark for 10 min. After being washed with PBS 3 times, the cells were observed under a fluorescence microscope (IX3; Olympus Corporation).

RNA extraction and reverse transcription-quantitative PCR (RT-qPCR)

TRIzol reagent (Roche Diagnostics) was used to extract total RNA, which was then reverse transcribed into cDNA using the RT kit (FSQ-101, Toyobo Co., Ltd.). The product was processed with SYBR-Green Premix Dimer Eraser (Takara Biotechnology Co., Ltd.), and the level of mRNA was then measured using the ABI 7000 sequence detection system (ABI PRISM^® 7000, Thermo Fisher Scientific, Inc.) (1 cycle, 95°C, 15 min; 40 cycles, 95°C, 10 sec, 60°C, 32 sec). GAPDH was used as the housekeeping gene for normalization. The primers and sequences used were as follows: IL-1β forward, 5′-CGA TCA CTG AAC TGC ACG CT-3′ and reverse, 5′-AGA ACA CCA CTT GTT GCT CCA-3′; GAPDH forward, 5′-AAGATC AAG ATC ATT GCT CCT CCT G-3′ and reverse, 5′-GCC GGA CTC GTC ATA CTC CT-3′; and PCSK9 forward, 5′-ACG ATG CCT GCC TCT ACT CC-3 and reverse, 5′-GCC TGT GAT GTC CCA CTC TGT-3′.

Enzyme-linked immunosorbent assay (ELISA)

ELISA kit (NeoBioscience, HongKong) was employed to measure the concentration of IL-1β secreted by HUVECs. Cells were first seeded into 24-well plates. Following the different treatments (oxLDL with/without PCSK9 siRNA, LV-PCSK9), the supernatant (100 µl) was collected for ELISA in accordance with the manufacturer's protocol (NeoBioscience Technology Company).

Western blot analysis

Cells were first washed thrice with cold PBS and then lysed with radioimmunoprecipitation assay buffer and phenylmethanesulfonyl fluoride (v/v, 94:6) on ice for 30 min. Lysed samples were centrifuged at 9,500 × g for 15 min at 4°C. The concentration of total proteins was determined using the BCA kit (Beijing CWBio). Protein samples (20 µg/well) were loaded for 8-12% sodium dodecyl sulfate polyacrylamide gel electrophoresis and then transferred onto a nitrocellulose filter membrane, which was blocked with 5% skimmed milk for 2 h at room temperature. Subsequently, the membrane was incubated with caspase-1 antibody (1:500; 22915-1-AP; ProteinTech Group, Inc.), IL-1β antibody (1:500; 16806-1-AP; ProteinTech Group, Inc.), IL-18 antibody (1:1,000; 10663-1-AP; ProteinTech Group, Inc.), NLRP3 antibody (1:500; A5652; ABclonal Biotech Co., Ltd.), GAPDH antibody (1:1,000; 10494-1-AP; ProteinTech Group, Inc.), PCSK9 antibody (1:500; 55206-1-AP; ProteinTech Group, Inc.), gasdermin D (GSDMD-N; 1:500; ab253350, Abcam), cleaved caspase-1 (1:1,000; ab207802; Abcam) and ubiquinol-cytochrome c reductase core protein 1 (UQCRC1) antibody (1:2,000; 21705-1-AP; ProteinTech Group, Inc.) diluted by the mixture of Tris-buffered saline (containing Tween-20) and 2.5% skimmed milk buffer at 4°C overnight. After washing 5 times with PBS containing Tween-20, the membrane was incubated with fluorescence-conjugated antirabbit IgG secondary antibody (1:2,000, SA00001-2; ProteinTech Group, Inc.) at room temperature for 2 h. After washing 5 times with Tris-buffered saline with Tween-20 (TBST; #9997; Cell Signaling Technology, Inc.), the optical density of the related bands was examined using Quantity One software V4.6.6 (Bio-Rad Laboratories, Inc.) with GAPDH as the internal reference.

Immunofluorescence

Following stimulation with various concentrations of oxLDL (50 and 100 µg/ml) for 24 h, the cells were fixed with 4% formaldehyde for 30 min. Following incubation with 0.1% Triton X-100 at room temperature for 30 min, the cells were blocked with goat serum for 1 h at room temperature. After being washed thrice with PBS, the cells were incubated with diluted PCSK9 antibody (1:100; #85813, Cell Signaling Technology, Inc.) at 4°C overnight. After being washed 5 times with PBS, the cells were incubated with Alexa Fluor-conjugated secondary antibody (1:50, A32731, Invitrogen; Thermo Fisher Scientific, Inc.) in the dark for 1 h at room temperature. Finally, the nuclei were stained with 4′,6-diamidino-2-phenylindole (Beyotime Institute of Biotechnology, Inc.) for 5 min at room temperature. After being washed 5 times with PBS, the cells were observed under a fluorescence microscope (IX3; Olympus Corporation).

Determination of ATP concentration in cells

Cells (1.0×10⁶/well) were washed with PBS twice and then lysed with 150 ml of lysis buffer. After being centrifuged at 1,500 × g for 5 min at 4°C, ATP detection reagent (Nanjing Jinagcheng Bioengineering Institute) was added, followed by examination using a microplate reader (iMark-03; Bio-Rad Laboratories, Inc.). ATP concentration was calculated using the standard curve.

Detection of ROS

The mitochondrial ROS probe (DCFH-DA; Nanjing KeyGen Biotech Co. Ltd.) was used to detect ROS production by HUVECs. Upon reaching 30-50% confluence, the cells were transfected with siRNA against PCSK9 and siUQCRC1. The transfected cells were incubated with oxLDL (100 µg/ml) for 24 h at room temperature. After discarding the medium, the cells were washed with cold PBS (pH 7.4) and then incubated with DCFH-DA at room temperature for 30 min. After being washed thrice with PBS, the cells were observed under a fluorescence microscope (IX3; Olympus Corporation).

Detection of mitochondrial membrane potential

The cells were first washed with PBS and then centrifuged at 1,500 × g for 5 min at room temperature. After discarding the supernatant, the cells were resuspended with 500 µl of JC-1 and then incubated at 37°C and 5% CO₂ for 15 min. Following centrifugation at 1,500 × g for 5 min at room temperature, the supernatant was removed and the cells were resuspended with 500 µl of 1X incubation buffer. The cells were then observed under a fluorescence microscope (IX3; Olympus Corporation) and examined by flow cytometry (Ex=488 nm; Em=530 nm) (Sysmex-Partec CyFlow^® Cube 6, Sysmex Partec). The ratio of green fluorescence intensity/red fluorescence intensity directly reflected mitochondrial membrane potential and mitochondrial function.

Statistical analysis

All data are presented as the means value with standard deviation. Differences between groups were determined by one-way ANOVA accompanied by the Tukey's or Bonferroni post hoc test (GraphPad Prism version 5.0; GraphPad, Inc.). A value of P<0.05 was considered to indicate a statistically significant difference.

Results

oxLDL induces HUVEC injury, pyroptosis and the release of inflammatory factors

HUVECs were incubated with oxLDL (50 and 100 µg/ml) for 24 h prior to detecting the expression levels of related molecules to explore whether oxLDL can cause damage to and the pyroptosis of these cells. oxLDL upregulated the protein expression levels of NLRP3, caspase-1 and IL-18 (Fig. 1A and B), and also increased the protein and mRNA expression of IL-1β (Fig. 1A-C). GSDMD-N expression was also increased by oxLDL stimulation (Fig. S1). The results of ELISA revealed that the IL-1β protein content increased in the supernatant of the HUVECs stimulated with oxLDL (Fig. 1D). The LDH level was also measured to determine the damage caused to the HUVECs by oxLDL. The data indicated that oxLDL promoted the release of LDH in a dose-dependent manner (Fig. 1E). Double staining with Hoechst 33342 and PI revealed that the ratio of PI-positive cells increased with the increasing oxLDL concentration (Fig. 1F and G). These results suggest that oxLDL induces injury to, and the pyroptosis of HUVECs, as well as the release of inflammatory factors from HUVECs.

Figure 1.

oxLDL induces the pyroptosis of HUVECs. (A and B) Expression of NLRP3, caspase-1, IL-1β and IL-18 proteins following stimulation with 50 or 100 µg/ml oxLDL for 24 h. Western blot analysis was used to measure protein expression. ^*P<0.05, ^**P<0.01 and ^***P<0.001 compared with the control group. (C) Expression of IL-1β mRNA in HUVECs stimulated with 50 or 100 µg/ml oxLDL for 24 h. ^*P<0.05 and ^**P<0.01 compared with the control group. (D) Concentration of IL-1β protein in supernatant of HUVECs stimulated with 50 or 100 µg/ml oxLDL for 24 h. ELISA was used to measure protein concentration. ^**P<0.01 and ^***P<0.001 compared with the control group. (E) LDH levels in the supernatant of HUVECs stimulated with 50 or 100 µg/ml oxLDL for 24 h. ^**P<0.01 and ^***P<0.001 compared with the control group. (F and G) Ratio of PI-positive HUVECs stimulated with 50 or 100 µg/ml oxLDL for 24 h. Propidium iodide (PI, red color). Original magnification, ×100; scale bar, 100 µm. ^***P<0.001 compared with the control group. All results are expressed as the means ± SD of 3 independent experiments. oxLDL, oxidized low-density lipoprotein; HUVECs, human umbilical cord endothelial cells; NLRP3, NLR family pyrin domain containing 3.

oxLDL upregulates PCSK9 protein expression in HUVECs

PCSK9 expression was examined following stimulation with oxLDL to examine the association between oxLDL and PCSK9. Western blot analysis revealed that oxLDL stimulation upregulated PCSK9 expression in HUVECs (Fig. 2A and B). Immunofluorescence also revealed similar findings, in which the PCSK9 fluorescence intensity increased following stimulation with oxLDL (Fig. 2C and D). These results indicate that oxLDL upregulates PCSK9 protein expression in HUVECs.

Figure 2.

oxLDL upregulates the expression of PCSK9 protein in HUVECs. (A and B) Expression of PCSK9 protein following stimulation with 50 or 100 µg/ml oxLDL for 24 h. Western blot analysis was used to measure protein expression. ^**P<0.01 and ^***P<0.001 compared with the control group. (C and D) Fluorescence intensity of PCSK9 in HUVECs stimulated with 50 or 100 µg/ml oxLDL. The nuclei were stained blue with 4′,6-diamidino-2-phenylindole (DAPI). Original magnification, ×100; scale bar, 75 µm. ^**P<0.01 and ^***P<0.001 compared with control group. All results are expressed as the means ± SD of 3 independent experiments. oxLDL, oxidized low-density lipoprotein; HUVECs, human umbilical cord endothelial cells; PCSK9, proprotein convertase subtilisin/kexin type 9.

siPCSK9 suppresses the damage, release of inflammatory factors and pyroptosis of HUVECs induced by oxLDL

It has been demonstrated that PCSK9 inhibition prevents inflammation and alleviates cell death (13). In the present study, the level of pyroptosis in the HUVECs was detected following stimulation with oxLDL with or without siPCSK9 transfection (50 µM siRNA). The siRNA efficiency of PCSK9 was determined (Fig. 3A). The expression levels of NLRP3, caspase-1 and IL-18 in the oxLDL group were significantly higher than those in the control group and siPCSK9 group (Fig. 3B and C). In addition, transfection with siPCSK9 decreased the protein and mRNA expression levels of IL-1β in the oxLDL group (Fig. 3B-D). The results of ELISA and LDH assay revealed that PCSK9 interference attenuated the upregulating effect of oxLDL on IL-1β expression (Fig. 3E) and reduced the damage to HUVECs caused by oxLDL, evidenced by the decreased activity of LDH (Fig. 3F). The PI staining data revealed that the pyroptosis of HUVECs was reduced by PCSK9 interference (Fig. 3G and H). These results suggest that PCSK9 interference suppresses the oxLDL-induced damage, the release of inflammatory substances and the pyroptosis of HUVECs.

Figure 3.

Interference with PCSK9 inhibits the oxLDL-induced pyroptosis of HUVECs. (A) Expression of PCSK9 protein in HUVECs following interference with PCSK9 (50 nM, 24 h) Western blot analysis was used to measure protein expression. ^***P<0.001 compared with the control group; NS, not significant. (B and C) Expression of NLRP3, caspase-1, IL-1β and IL-18 protein in HUVECs following interference with PCSK9 following stimulation with 100 µg/ml oxLDL for 24 h. Western blot analysis was used to measure protein expression. ^**P<0.01 and ^***P<0.001 compared with the control group; ^#P<0.05, ^##P<0.01 and ###P<0.001 compared with the oxLDL group. (D) Expression of IL-1β mRNA. ^***P<0.001 compared with the control group; ^#P<0.05 compared with the oxLDL group. (E) Concentration of IL-1β in supernatant of HUVECs. ^**P<0.01 compared with the control group; ^#P<0.05 compared with the oxLDL group. (F) LDH levels in supernatant of HUVECs. ^***P<0.001 compared with the control group; ^#P<0.05 compared with the oxLDL group. (G and H) Ratio of PI-positive HUVECs. Original magnification, ×100; scale bar, 100 µm. ^***P<0.001 compared with the control group; ^##P<0.01 compared with the oxLDL group. All results are expressed as the means ± SD of 3 independent experiments. oxLDL, oxidized low-density lipoprotein; HUVECs, human umbilical cord endothelial cells; PCSK9, proprotein convertase subtilisin/kexin type 9.

Overexpression of PCSK9 induces the pyroptosis of HUVECs

HUVECs were transfected with LV-PCSK9 to explore the association between PCSK9 and pyroptosis. The mRNA and protein levels of PCSK9 significantly increased following transfection with LV-PCSK9 (Fig. 4A and B). In addition, NLRP3, caspase-1 cleaved caspase-1 and IL-1β protein expression increased following the overexpression of PCSK9 (Fig. 4C and D). The protein and mRNA expression of IL-1β (Fig. 4E and F) and the LDH level (Fig. 4G) in the supernatant increased following the overexpression of PCSK9. In addition, the ratio of PI-positive cells significantly increased following the overexpression of PCSK9 (Fig. 4H and I). These results indicate that PCSK9 overexpression induces the pyroptosis of HUVECs.

Figure 4.

Overexpression of PCSK9 induces the pyroptosis of HUVECs. (A and B) Expression of PCSK9 (A) mRNA and (B) protein in HUVECs following transfection with LV-PCSK9. ^***P<0.001 compared with LV-NC group. (C and D) Expression of NLRP3, caspase-1 and IL-1β proteins in HUVECs. ^***P<0.001 compared with the LV-NC group. (E) Concentration of IL-1β in the supernatant of HUVECs determined by ELISA. ^**P<0.01 compared with the LV-NC group. (F) Expression of IL-1β mRNA in HUVECs. ^**P<0.01 compared with the LV-NC group. (G) LDH levels in the supernatant of HUVECs. ^**P<0.01 compared with the LV-NC group. (H and I) Ratio of PI-positive HUVECs. PI is shown in red. Original magnification, ×100; scale bar, 100 µm. ^***P<0.001 compared with the LV-NC group. All results are expressed as the means ± SD of 3 independent experiments. oxLDL, oxidized low-density lipoprotein; HUVECs, human umbilical cord endothelial cells; PCSK9, proprotein convertase subtilisin/kexin type 9.

PCSK9 overexpression increases ROS generation and induces mitochondrial dysfunction by inhibiting UQCRC1

Excessive ROS production caused by mitochondrial dysfunction can promote the pyroptosis of endothelial cells (9) and reduce ATP production (14). In the present study, the intracellular ROS levels were detected following transfection with LV-PCSK9 to determine whether PCSK9 promotes the pyroptosis of endothelial cells through the ROS pathway. PCSK9 overexpression elevated the ROS levels (Fig. 5A and B) and inhibited ATP production (Fig. 5C). UQCRC1 is a subunit of mitochondrial respiratory chain complex III and maintains mitochondrial function (14). UQCRC1 expression was thus examined following the overexpression of PCSK9 in order to determine the association between PCSK9 and UQCRC1. The data indicated that PCSK9 overexpression inhibited the protein expression of UQCRC1 (Fig. 5D). These results suggest that PCSK9 increases ROS production and causes mitochondrial disorder by inhibiting UQCRC1.

Figure 5.

Overexpression of PCSK9 increases ROS production and inhibits ATP production. (A and B) ROS level in HUVECs following transfection with LV-PCSK9. Original magnification, ×100; scale bar, 100 µm. ^***P<0.001 compared with the LV-NC group. (C) ATP concentration in HUVECs following transfection with LV-PCSK9. ^*P<0.05 compared with the LV-NC group. (D) Expression of UQCRC1 protein in HUVECs following transfection with LV-PCSK9. ^**P<0.01 compared with the LV-NC group. All results are expressed as the means ± SD of 3 independent experiments. oxLDL, oxidized low-density lipoprotein; HUVECs, human umbilical cord endothelial cells; PCSK9, proprotein convertase subtilisin/kexin type 9; ROS, reactive oxygen species; ATP, adenosine triphosphate.

siPCSK9 reverses mitochondrial dysfunction induced by oxLDL by recovering UQCRC1 expression

HUVECs were incubated with siPCSK9 for 24 h and then stimulated with oxLDL (100 µg/ml) to investigate whether oxLDL inhibits UQCRC1 expression through PCSK9. The data indicated that UQCRC1 expression was significantly increased following transfection with siPCSK9 compared with that after oxLDL stimulation alone (Fig. 6A). The ROS level significantly decreased following transfection with siPCSK9, but was significantly increased following transfection with siPCSK9 and siUQCRC1 (the transfection efficiency is shown in Fig. 6B and C) compared with that following transfection with siPCSK9 only (Fig. 6D and E). The cell membrane potential level was also examined by JC-1 staining. The green fluorescence was higher in the oxLDL group than in the control group and recovered compared with that in the siPCSK9-transfected group. However, transfection with siPCSK9 and siUQCRC1 enhanced the intensity of green fluorescence (Fig. 6F). Flow cytometry revealed that the area in the Q2 quadrant decreased, whereas the areas in the Q3 and Q4 quadrants increased following oxLDL stimulation. PCSK9 interference increased the area in Q2 quadrant. The simultaneous interference of PCSK9 and UQCRC1 decreased the area in the Q2 quadrant, but increased the areas in the Q3 and Q4 quadrants (Fig. 6F and G). These results indicate that oxLDL inhibits UQCRC1 expression, promotes mitochondrial membrane potential collapse, and damages mitochondrial function, and these processes can be reversed PCSK9 interference.

Figure 6.

Interference with PCSK9 attenuates excessive production of ROS. (A) Expression of UQCRC1 following stimulation with oxLDL (100 µg/ml), and the effect of interference with PCSK9 expression. ^**P<0.01 compared with the control group; ^##P<0.01 compared with the oxLDL group. (B and C) Expression of UQCRC1 protein in HUVECs following interference with UQCRC1 (50 nM, 24 h). Western blot analysis was used to measure protein expression. ^**P<0.01 compared with the control group. (D and E) Mitochondrial ROS level following stimulation with oxLDL (100 µg/ml), and the effect of interference with PCSK9 expression and UQCRC1 expression. Original magnification, ×200; scale bar, 20 µm. ^***P<0.001 compared with the control group; ^##P<0.01 compared with the oxLDL group; ^¥¥P<0.01 compared with the oxLDL + siPCSK9 group. (F) Changes in mitochondrial membrane potential as observed by fluorescence microscopy. Original magnification, ×100; scale bar, 100 µm. (G) Mitochondrial membrane potential levels measured by flow cytometry. ^***P<0.001 compared with the control group; ^##P<0.01 compared with the oxLDL group; ^¥P<0.05 compared with the oxLDL + siPCSK9 group. All results are expressed as the means ± SD of 3 independent experiments. oxLDL, oxidized low-density lipoprotein; HUVECs, human umbilical cord endothelial cells; PCSK9, proprotein convertase subtilisin/kexin type 9; UQCRC1, ubiquinol-cytochrome c reductase core protein 1.

Discussion

The present study demonstrated that PCSK9 interference attenuated HUVEC pyroptosis induced by oxLDL. On the one hand, oxLDL induced the pyroptosis of HUVECs in a concentration-dependent manner and upregulated PCSK9 expression. PCSK9 interference relieved the pyroptosis induced by oxLDL. On the other hand, PCSK9 overexpression promoted pyroptosis, UQCRC1 downregulation and ROS generation. siPCSK9 can alleviate the inhibition of UQCRC1 expression by oxLDL, ROS production, and the disorder of mitochondrial membrane potential (Fig. 7).

Figure 7.

Schematic diagram of the regulatory effects of PCSK9 on endothelial cell pyroptosis by increasing ROS production via the inhibition of UQCRC1. PCSK9 leads to mitochondrial dysfunction through the inhibition of the subunit UQCRC1, and this promotes the production of ROS. ROS improves the action of NLRP3, which recruits caspase-1 and promotes inflammatory cytokine maturation and cellular pore formation. PCSK9, proprotein convertase subtilisin/kexin type 9; UQCRC1, ubiquinol-cytochrome c reductase core protein 1; ROS, reactive oxygen species; NLRP3, NLR family pyrin domain containing 3.

AS is a chronic inflammatory disease accompanied by endothelial dysfunction and lipid deposition (1). Endothelial dysfunction is the initial cause of AS. In AS lesions, endothelial cells are often accompanied by pyroptosis (15), which is associated with the activation of related inflammatory bodies (NLRP3 and caspase-1) and the release of inflammatory factors (IL-1β and IL-18) and cell dysfunction to stimulate the recruitment of immune cells and then promote AS (16). However, the mechanisms underlying pyroptosis remain unclear. NLRP3 and pyrin in cells can recruit inflammatory bodies to form mature bodies, thus leading to the maturation of inflammatory factors and the activation of gasdermin family (17). ROS can activate NLRP3 and mediate pyroptosis (18). In the present study, PCSK9 overexpression induced pyroptosis and increased ROS production.

PCSK9 is involved in the pathogenesis of AS by regulating lipid metabolism (19,20) as confirmed by the clinical lipid-lowering effect of PCSK9 inhibitors (21). This enzyme binds to LDLR, promotes its intracellular degradation, and then reduces LDLR level and LDL-C metabolism (19). PCSK9 is also involved in cell death and inflammation. Low shear stress can promote the expression of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) by activating ROS and NF-κB, and the upregulation of LOX-1 can upregulate PCSK9 expression and vice versa (11). In the present study, PCSK9 overexpression promoted the pyroptosis of endothelial cells, and its interference alleviated the pyroptosis induced by oxLDL. These results suggest that PCSK9 is an important mediating factor in pyroptosis and ROS production. However, the mechanisms through which PCSK9 upregulates ROS expression have been rarely reported, at least to the best of our knowledge.

The mitochondria can regulate cell function by releasing signal molecules (10). Excessive inflammatory stimulation and oxidative stress can promote the disorder of mitochondrial regulation (22). oxLDL inhibits mitochondrial gene expression (14), induces mitochondrial dysfunction, and promotes AS (23). Moreover, PCSK9 is closely related to mitochondrial function and oxidative damage (24,25), promotes the mitochondrial DNA damage of endothelial cells, and facilitates the occurrence of apoptosis (26). However, the association between PCSK9 and mitochondrial damage remains unclear. UQCRC1 is a subunit of mitochondrial complex III, and its interference in endothelial cells can lead to excess ROS production (14). The present study revealed that PCSK9 inhibited the function of mitochondrial complex III by downregulating UQCRC1, leading to ROS elevation. This finding also suggests that PCSK9 is an important mediator of the oxidative damage mediated by oxLDL through mitochondrial-related DNA. Elevated ROS levels have a pathological potential in leading mtDNA mutation and oxidative damage to the respiratory chain (27), which also increased following the overexpression of PCSK9 in the present study. Therefore, the inhibition of UQCRC1 may result from the ROS level regulated by PCSK9. However, ROS elevation also upregulates PCSK9 expression and damages mitochondrial genes (11). Therefore, ROS and PCSK9 can be regulated by mitochondrial genes (such as UQCRC1) and then participate in the occurrence and development of AS.

The present study has some limitations. First, the extra-cellular level of PCSK9 was not detected under the effect of oxLDL. Second, the mechanism through which PCSK9 inhibits the expression of UQCRC1 was not elucidated. Third, the effect of UQCRC1 defect was only observed in vitro, and in vivo experiments were not conducted. These limitations also provide directions for future studies.

In conclusion, the present study demonstrates that oxLDL promotes the pyroptosis of HUVECs and simultaneously upregulates PSCK9 expression, leading to mitochondrial dysfunction and ROS production resulting from UQCRC1 inhibition. However, PCSK9 interference blocks oxLDL-induced UQCRC1 inhibition, pyroptosis and mitochondrial ROS production in HUVECs. These results indicate that UQCRC1 plays a potential role in the antioxidant response and AS treatment.

Funding Statement

The present study was supported by the Natural Science Foundation of China (grant no. 81970389) and the Natural Science Foundation of Hunan Province China (grant nos. 2019JJ50531 and 2020JJ4541).

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Authors' contributions

LL and ZW contributed to the conception and design of the study. JZ, JT and LX performed the experiments, collected the data and analyzed the data and assisted in the interpretation of the results. All authors read and approved the final manuscript.

Ethics approval and consent to participate

Not applicable.

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.