Fig. 6.

PPD-specific effector cytokine producing parenchymal CD4⁺ T cells and CD8⁺ T cells in the lungs of naïve and vaccinated mice at 4 weeks post vaccination. Mice were vaccinated as shown in Fig. 3a and sacrificed at 4 weeks post vaccination. Fluorochrome-conjugated CD45.2 was injected intravenously before sacrificing. Lungs cells were stimulated ex-vivo with PPD-T (PPD). The stimulated cells were analysed using flow cytometry (Appendix 3). Total cell number of PPD-specific IFN-γ and/or TNF-α producing lung parenchymal CXCR3⁺ KLRG1^- CD4⁺ T cells (a) and CD8⁺ T cells (b) in naïve and vaccinated mice. Each symbol represents one animal and the line is the median of each group (n = 4–6 mice). Data are representative of two independent experiments. Statistical significance was determined using Kruskal-Wallis test with Dunn's multiple comparisons test. *p ≤ 0.05; **, p ≤ 0.01.