Table 1.
Different lipid classes, with the corresponding resonance assignments, were identified by the NMR analysis. DAGP = diacylglycerophospholipids; FA = fatty acid chain; MUFA = monounsaturated fatty acids; SL = sphingolipids; PC = phosphatidylcholine; PG = phosphatidylglycerol; PUFA = polyunsaturated fatty acids; TAG = triacylglycerol; UFA = unsaturated fatty acids.
| Resonance | 1H NMR Signal | Chemical Shift (ppm) | Lipid Class |
|---|---|---|---|
| 1 | –C18H3 | 0.69 | Cholesterol |
| 2 | –CH3 | 0.89 | Total FA |
| 3 | –C19H3 | 1.02 | Cholesterol |
| 4 | –(CH2)n– | 1.25 | Total FA |
| 5 | =CHCH2CH2(CH2)– | 1.30 | Total FA |
| 6 | –CO-CH2CH2– | 1.62 | Total FA |
| 7 | –CH2HC= | 2.02 | UFA |
| 8 | –CH2HC= | 2.07 | UFA |
| 9 | –CO-CH2– | 2.37–2.23 | Total FA |
| 10 | =CHCH2CH= | 2.74 | n-6 PUFA |
| 11 | =CHCH2CH= | 2.88 | n-3 PUFA |
| 12 | –N+(CH3)3 | 3.35–3.15 | PC/PE/SL |
| 13 | CH2CHCH2 | 3.74 | PG |
| 14 | PC and DAGP signals | 4.5–3.5 | PC |
| 15 | >C2H in glycerol backbone | 5.21 | DAGP |
| 16 | >C2H in glycerol backbone | 5.26 | TAG |
| 17 | –HC=CH– in fatty acyl chain PUFA and MUFA | 5.42–5.29 | UFA |
| 18 | OH-CH-CH=CH- | 5.74 | SL |