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. 2021 Jan 22;11(2):142. doi: 10.3390/biom11020142

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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DNA methylation of BDNF in probiotic-treated and untreated Zebrafishes in the gut and brain. (A) Average methylation (%) of BDNF in the brain of untreated (CTRL) and probiotic-treated (PROBIO) Zebrafishes. Statistical analysis was performed using Student t-test. (B) Methylation level (%) at single-CpG sites was shown for the brain of CTRL and PROBIO groups. (C) BDNF average methylation (%) in the gut of untreated (CTRL) and probiotic-treated (PROBIO) Zebrafishes. Statistical analysis was performed using Student t-test. (D) Single-CpG methylation (%) at the BDNF promoter in the gut of CTRL and PROBIO groups. Statistical analyses were performed using one-way ANOVA followed by Tukey’s multiple comparison post-hoc test.