Table 4.
Interactive and main effects of cadmium (Cd) and zinc (Zn) on the concentrations of hydrogen peroxide (H2O2) and myeloperoxidase (MPO) in the brain tissue of male rats 1,2.
| Parameter | 5 mg Cd/L + 30 mg Zn/L | 5 mg Cd/L + 60 mg Zn/L | ||||||
|---|---|---|---|---|---|---|---|---|
| Main Effect of Cd |
Main Effect of Zn |
Interactive Effect of Cd + Zn |
Cd + Zn Effect vs. Cd Effect + Zn Effect Character of Cd-Zn Interaction |
Main Effect of Cd |
Main Effect of Zn |
Interactive Effect of Cd + Zn |
Cd + Zn Effect vs. Cd Effect + Zn Effect Character of Cd-Zn Interaction |
|
| H2O2 | 29.81 ‡ | 20.42 ‡ | 63.25 ‡ | 0 vs. +60 3 + 0 0 vs. +60 Antagonism |
50.14 ‡ | 10.95 † | 36.89 ‡ | 0 vs. +60 + 0 0 vs. +60 Antagonism |
| MPO | 19.59 ‡ | 71.50 ‡ | 51.54 ‡ | −13 vs. +37 + 0 −13 vs. +37 4 |
NS | 73.27 ‡ | 75.84 ‡ | −27 vs. +37 + 0 −27 vs. +37 4 |
1 The outcomes of the ANOVA/MANOVA statistical test are expressed as F values and the level of statistical significance (p). F values having p < 0.05 are recognized statistically significant (where † p < 0.01, ‡ p < 0.001). NS—a lack of statistically significant effect (p > 0.05). 2 To describe the possible character of the interaction between Cd and Zn, the effect of co-administration of the two elements was compared to the sum of the effects of their action in the case of separate administration (Cd + Zn 30 effect vs. Cd effect + Zn 30 effect and Cd + Zn 60 effect vs. Cd effect + Zn 60 effect). The effects of Cd, Zn 30, Zn 60, Cd + Zn 30, and Cd + Zn 60 are shown as percentage differences (−, decrease; +, increase) vs. the control. The interaction between Cd and Zn (Cd-Zn 30 or Cd-Zn 60) was considered as antagonistic (antagonism) when the effect of co-administration of Cd and Zn (expressed as a percentage difference vs. the control) was below the sum of the effects noted in the case of their separate administration. 3 The values express percentage differences. 4 The description of the character of the interaction between Cd and Zn was impossible.