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. 2021 Jan 23;11(2):147. doi: 10.3390/biom11020147

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Gene expression profile in male and female bladder SMCs. SMCs were separated from the epithelial layer as mentioned in the “Materials and Methods” section. RNA was isolated from the SMC layer and the expression of the epithelial cell marker Krt7 and the SMC marker Myh11 (A), P2Y receptors (B), P2X receptors (C), ectonucleotidases (D), and P1 receptors (E) were quantified by RT-qPCR. Data are normalized to GAPDH (glyceraldehyde 3-phosphate dehydrogenase; left panels) and ACTB (actin beta; right panels) mRNA level. Entpd1^+/+: wild type mice. Entpd1^−/−: NTPDase1 deficient mice. Data presented are the means ± SEM of three independent experiments in duplicate each with SMCs pooled from three mice.