Figure 2.

Levels of infectious particles in cell cultures treated with fluoxetine (FLX) before, during, or after inoculation of SARS-CoV-2. Vero E6 cells were seeded in 24-well plates at 10⁵ cells per well. The cells were inoculated with SARS-CoV-2 (black circles), SARS-CoV-2 + 10 μM of FLX (black squares), or SARS-CoV-2 incubated for 2 h and then treated with 10 μM of FLX (white circles), or the cells were incubated for 2 h in presence of 10 μM of FLX, followed by inoculation with SARS-CoV-2 and incubation for 2 h (black triangles). For each condition, the MOI was 0.01 and the cells were washed three times with complete medium after incubation and then treated again with 10 μM of FLX, followed by re-incubation. Levels of infectious particles in supernatants were determined using the endpoint dilution assay at days 1, 2, 3, 5 post-infection. The Spearman–Karber method was used to determine the tissue culture 50% infectious dose (TCID₅₀/mL). The results are presented as the mean ± SD of three independent experiments.