Figure 7.

5-FU inhibited the tumoroid growth of mCRC, although not completely: (A) a scheme of the experimental protocol used for panel (B–G). LuM1/m9 cells were cultured with 5-FU at 0, 1, or 10 μM for 3 days in a medium containing 10% FBS; (B) representative images of cell aggregates of LuM1/m9. Scale bars, 200 μm²; (C) column scatters plotting of tumoroid size. Representative data from a single well were shown; (D) the average size of cell aggregates. n = 4 independent culture wells; (E) the rate of tumoroids larger than 300 μm². n = 4 independent culture wells; (F) Mmp9 promoter activity. n = 4 independent culture wells; and (G) cell viability. n = 4 independent culture wells. * p < 0.05, ** p < 0.01, **** p < 0.0001.