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. 2021 Feb 9;10(2):356. doi: 10.3390/cells10020356

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Lyso-Gb3 and Gb3 effect on HUVECs. Intracellular ROS levels and cell viability were assayed in HUVECs treated with TBH 60 µM, Lyso-Gb3 25 nM, and Gb3 10 µM. Intracellular ROS were measured as fluorescence intensity, using a DCFH-DA probe. The results, in arbitrary units (au), are presented as mean ± SD of three different experiments analyzed in triplicate (a). Cell viability was evaluated with MTS assay. The results presented as mean % of viable cells ± SD of three different experiments analyzed in triplicate (b). Comparison among multiple groups was analyzed by one-way analysis of variance, followed by Bonferroni’s post hoc test. * p < 0.01.