Table 3.
Summary of the antibodies and experimental conditions used in immunofluorescence analysis.
| Marker | Blocking | Primary Antibody | Dilution | Secondary Antibody | Dilution |
|---|---|---|---|---|---|
| Pan Cytokeratin | 10% GS + 0.5% Triton X-100 | ThermoFisher Scientific, #MA5-12231, Mouse monoclonal |
1:500 | Alexa Fluor® 488 ThermoFisher Scientific,#A-11001 Goat anti-mouse |
1:500 |
| Vimentin | 10% GS + 0.5% Triton X-100 | ThermoFisher Scientific, #MA3745 Mouse Monoclonal |
1:100 | Alexa Fluor® 488 ThermoFisher Scientific,#A-11001 Goat anti-mouse |
1:500 |
| MEF2C | 10% GS + 0.5% Triton X-100 | ThermoFisher Scientific, #PA5-28247, Rabbit polyclonal |
1:100 | Alexa Fluor® 555 ThermoFisher Scientific, #A-21428 Goat anti-rabbit | 1:250 |
| Ki-67 | 3% BSA + 0.5% Triton X-100 | ThermoFisher Scientific #PA5-19462, Rabbit |
1:100 | Alexa Fluor® 555 ThermoFisher Scientific, #A-21428 Goat anti-rabbit |
1:500 |
| Ki-67 | 10% GS + 0.5% Triton X-100 | Santa Cruz Biotechnology #sc-7846 Goat Polyclonal |
1:100 | IgG-FITC Santa Cruz Biotechnology, #sc-2024, donkey anti-Goat |
1:500 |
| VEGFR-2 | 3% BSA + 0.5% Triton X-100 | ThermoFisher Scientific, #MA5-15556, Mouse monoclonal |
1:250 | Alexa Fluor® 488 ThermoFisher Scientific, #A-11001 Goat anti-mouse |
1:500 |
| ß-catenin | 10% GS + 0.5% Triton X-100 | ThermoFisher Scientific, #71-2700, Rabbit polyclonal |
1:50 | Alexa Fluor® 488 ThermoFisher Scientific, #A-21206 donkey anti-rabbit |
1:500 |