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. 2021 Feb 13;10(2):202. doi: 10.3390/pathogens10020202

Figure 2.

Figure 2

TPP suppresses PRRSV infection regardless of its pre-treatment and post-treatment. (A,C) Marc-145 cells were pre-treated with different concentrations of TPP for 2 h and then infected with PRRSV-CHR6 for 36 h, and the expression of viral ORF7 (PRRSV N) was detected by qRT-PCR (A) and viral N protein was determined by Western blot analysis (C). (B,D) Marc-145 cells were infected with PRRSV-CHR6 (MOI = 0.6) for 4 h, then TPP was added, cells were incubated for another 36 h, and the expression of viral ORF7 (PRRSV N) was detected by qRT-PCR (B) and N protein was determined by Western blot (D). Data are representative of the results of three independent experiments (means ± SE). Significant differences compared to the control group are denoted by *** p < 0.001.