Figure 3.

TPP inhibits PRRSV attachment, entry, replication, and release. Marc-145 cells were infected with PRRSV-CHR6 at an MOI of 6, and the infected cells were cultured in the presence of various concentrations of TPP and collected at indicated time points post-infection to determine viral N protein levels by Western blot. (A) Different TPP treatment schemes. Red bars represent the PRRSV infection period, green bars represent TPP treatment, magenta vertical bars represent removing TPP, and purple vertical bars represent cell harvest. (B–E) Viral binding, internalization, replication, and release were performed in cells treated, as described in (A), and then cells were harvested for Western blot analysis. Data are representative of the results of three independent experiments.