Table 1.
Mutant Strain | Storage# | Gene | Mutation, Reference to Genome of PB1 (acc no NC_010634) | Sensitivity to | ||
---|---|---|---|---|---|---|
fPS-2 | fPS-65 | fPS-90 | ||||
M1-fps2-wt | 6582 | galU | del-TAAAAGAGATCA, 1,869,879 | Res * | Sens ** | Res |
M2-fps2-wt | 6583 | galU | del-TAAAAGAGATCA, 1,869,879 | Res | Sens | Res |
M3-fps2-wt | 6584 | galU | del-TAAAAGAGATCA, 1,869,879 | Res | Sens | Res |
M1-fps90-wt | 6585 | galU | del-TAAAAGAGATCA, 1,869,879 | Res | Sens | Res |
M3-fps90-wt | 6586 | galU | del-AAGAGATCAAAAAT, 1,869,882 | Res | Sens | Res |
M4-fps90-wt | 6587 | ompF | C → CT, 1,721,035 | EOP *** | Sens | Res |
M5-fps90-wt | 6650 | galU | del-AAAGAG, 1,869,881 | Res | Sens | Res |
M6-fps90-wt | 6651 | galU | del-AAAGAG, 1,869,881 | Res | Sens | Res |
M7-fps90-wt | 6652 | galU | ca. 7.9 kb del (1,870,248..1,878,146) | Res | Sens | Res |
M8-fps90-wt | 6653 | galU | del-TAAAAGAGATCA, 1,869,879 | Res | Sens | Res |
M9-fps90-wt | 6654 | ompF | promoter region, T → C, 1,721,588 | EOP | Sens | Res |
M10-fps90-wt | 6655 | galU | ca. 4.3 kb del (1,865,646..1,869,969) | Res | Sens | Res |
M1-fps65-wt | 6941 | hldE | 147 bp del (3,957,759..3,957,905) | Res | Res | Res |
M2-fps65-wt | 6942 | hldD | 517 bp del (61,956..62,473) | Res | Res | Res |
M3-fps65-wt | 6943 | hldE | 147 bp del (3,957,759..3,957,905) | Res | Res | Res |
* Res: resistant; ** Sens: sensitive; *** EOP: 104-fold reduced EOP.