Table 1.

Summary of multiple reaction monitoring (MRM) (m/z), retention time (RT), Q1 pre-rod potential, collision energy (CE), Q3 pre-rod potential, and dynamic range of all 66 eicosanoids and internal standards (IS).

Analytes ID	Analytes	MRM (m/z)	RT (Min.)	Q1 Pre Vias (V)	CE (V)	Q3 Pre Vias (V)	Dynamic Range (ng/mL)
1	PGJ2	333.50 > 233.40	13.7	12	10	15	0.01–200
3	20-OH-PGE2	367.40 > 287.45	4.9	12	17	19	0.01–200
4	PGB2	333.50 > 235.45	13.9	11	19	15	0.01–200
6	PGD2	351.50 > 271.50	12.0	12	17	17	0.01–200
7	PGE2	351.50 > 271.50	11.6	12	17	17	0.01–200
8	AA	303.50 > 259.50	20.6	22	14	11	1–200
9	15-HETE	319.50 > 219.45	17.3	11	14	13	0.01–200
10	12-HETE	319.50 > 179.35	17.7	11	15	10	0.01–200
11	11-HETE	319.50 > 167.45	17.5	11	16	10	0.01–200
12	8-HETE	319.50 > 155.35	17.7	16	14	24	0.01–200
13	5-HETE	319.50 > 115.35	18.0	11	14	16	0.01–200
14	LTE4	438.40 > 235.45	12.8	10	23	14	1–200
15	LTD4	495.50 > 177.35	11.0	11	20	10	1–200
16	LTC4	624.50 > 272.40	12.0	22	24	12	1–200
17	LTB4	335.50 > 195.40	14.7	12	16	11	0.01–200
18	13,14-DiOH-15-Keto-PGE2	351.40 > 333.50	12.9	11	12	10	0.01–200
19	11-B-PGF2	353.40 > 193.40	10.1	12	24	12	0.01–200
20	8-iso-PGF2	353.40 > 193.40	9.8	12	24	12	0.01–200
21	PGF2	353.40 > 193.40	10.9	12	24	12	0.01–200
22	15-Keto-PGE2	349.30 > 235.40	12.3	18	15	15	0.01–200
23	6-Keto-PGF1	369.40 > 245.45	7.7	12	25	15	0.01–200
24	TXB2	369.50 > 169.40	10.0	13	18	10	0.01–200
29	13,14-DiOH-PGF2	355.40 > 311.50	12.0	11	25	14	0.01–200
30	PGF1	355.40 > 311.50	11.0	11	25	14	0.01–200
31	13,14-DiOH-15-Keto-PGF2	353.50 > 113.40	12.7	12	25	11	0.01–200
32	13,14-DiOH-15-Keto-PGE1	353.40 > 335.50	13.2	12	13	22	0.01–200
33	PGD1	353.50 > 317.50	12.1	12	17	15	0.01–200
34	13,14-DiOH-PGE1	355.30 > 337.55	12.4	11	16	11	0.01–200
35	TXB3	367.50 > 169.35	8.6	10	17	10	0.01–200
36	15-deoxy-delta 12,14 PGJ2	315.50 > 271.50	13.8	12	14	17	0.01–200
37	PGE1	353.50 > 317.50	11.8	12	17	15	0.01–200
38	PGE3	349.50 > 269.50	10.2	12	16	17	0.01–200
39	PGD3	349.50 > 269.50	10.7	12	16	17	0.01–200
40	PGF3	351.50 > 307.50	9.5	12	18	14	0.1–200
41	14,15-LTC4	624.50 > 272.40	13.0	22	23	18	1–200
42	Tetranor-PGEM	327.40 > 309.45	2.2	11	12	21	0.1–200
43	Tetranor-PGFM	329.40 > 311.50	1.8	16	14	10	0.1–200
44	11-De TXB3	365.40 > 303.50	10.4	13	16	20	0.01–200
45	2,3 Dinor 8-iso PGF2	325.50 > 237.50	7.2	12	13	15	0.01–200
53	Docosahexaenoic Acid	327.50 > 283.55	20.3	11	12	18	1–200
54	9(10)-DiHOME	313.50 > 201.40	15.2	11	22	12	0.01–200
55	12(13)-DiHOME	313.50 > 183.40	15.1	11	22	11	0.01–200
56	4-HDHA	343.50 > 101.30	18.1	11	14	29	0.01–200
57	7-HDHA	343.50 > 141.30	17.7	12	13	23	0.01–200
58	8-HDHA	343.50 > 109.30	17.8	11	20	30	0.01–200
59	10-HDHA	343.50 > 153.40	17.5	12	15	25	0.01–200
60	11-HDHA	343.50 > 121.35	17.7	12	15	18	0.01–200
61	11-HEDE	323.40 > 199.45	18.5	11	21	12	0.01–200
62	15-HEDE	323.40 > 223.50	18.6	11	20	14	0.01–200
63	5-HEPE	317.50 > 115.30	17.0	12	15	18	0.01–200
64	8-HEPE	317.50 > 155.35	16.7	10	14	26	0.01–200
65	11-HEPE	317.50 > 167.45	16.6	11	15	10	0.01–200
66	12-HEPE	317.50 > 179.40	16.8	12	14	10	0.01–200
67	15-HEPE	317.50 > 219.45	16.7	12	13	13	0.01–200
68	9(S)-HOTrE	293.50 > 171.35	16.2	12	15	10	0.01–200
69	13(S)-HOTrE	293.50 > 224.45	16.3	11	14	13	0.01–200
70	5-OxoETE	317.50 > 203.40	18.6	10	18	12	0.01–200
71	12-OxoETE	317.50 > 153.40	18.0	16	16	25	0.01–200
72	15-OxoETE or 15-KETE	317.50 > 113.35	17.7	12	20	18	0.01–200
73	9-OxoODE or 9-KEDE	293.50 > 185.40	17.8	11	21	11	0.01–200
74	15-OxoEDE or 15-KEDE	321.50 > 223.45	19.1	11	23	14	0.01–200
76	Tetranor-PGDM	327.40 > 309.45	2.8	11	13	20	0.1–200
77	Tetranor-PGJM	309.30 > 155.35	4.2	12	22	24	0.01–200
78	Resolvin D1	375.60 > 141.30	12.9	10	15	23	1–200
79	Resolvin D2	375.60 > 175.35	12.2	10	23	10	0.1–200
80	Resolvin D3	375.60 > 147.40	12.0	10	22	23	0.1–200
25	TXB2-d4	373.50 > 173.40	10.0	13	19	10	NA
26	PGE2-d4	355.50 > 275.55	11.5	13	17	18	NA
27	AA-d8	311.40 > 267.55	20.6	10	13	17	NA
46	15 -HETE-d8	327.50 > 226.50	17.2	16	13	14	NA
47	LTB4-d4	339.50 > 197.45	14.7	10	16	12	NA
81	Resolvin D1-d5	380.50 > 141.30	12.8	13	16	22	NA

NA: Not Applicable.