Figure 6.

EGCG treatment reduced liver macrophage infiltration and modulated M1/M2 polarization in NAFLD mice. Diet-induced NAFLD mice were randomly divided into three treatment groups: PBS, EGCG-25, and EGCG-50, and treated for 8 weeks. Intrahepatic macrophages were immunophenotyped using flow cytometry. The M1 subpopulation was defined as either CD45⁺F4/80⁺Ly6C⁺ or CD45⁺F4/80⁺MHC-II⁺, while CD45⁺F4/80⁺CD206⁺ and CD45⁺F4/80⁺CD23⁺ cells were considered as M2 macrophages. (a) The sequential gating strategy used for intrahepatic immune cell analysis; (b) total CD45⁺ cells; (c) CD45⁺F4/80⁺ cells; (d) CD45⁺F4/80⁺CD206 M2 cells; (e) CD45⁺F4/80⁺CD23⁺ M2 cells; (f) CD45⁺F4/80⁺MHC-II⁺ M1 cells; and (g) CD45⁺F4/80⁺Ly6C⁺ M1 cells. All cell numbers shown are the number of cells per mouse liver.