Table 1.
SAXS structural parameters of the A3BCTD* and A3BCTD*/dZ-oligo complex.
| Structural Parameters | A3BCTD* | A3BCTD*/dZ-Oligo | A3BCTD* Dimer | Species 1 a (A3BCTD* Dimer) | Species 2 a (A3BCTD*/dZ-Oligo) |
|---|---|---|---|---|---|
| Guinier analysis | |||||
| I(0) (cm−1) | 0.00940 (5) | 0.01031 (5) | 0.00480 (4) | 0.0036 (1) | 0.00110 (5) |
| Rg (Å) | 18.8 (2) | 19.8 ± 0.2 | 24.2 (8) | 24.8 (1.5) | 20.0 (1.5) |
| qmin (Å−1) | 0.0106 | 0.0106 | 0.0165 | 0.01795 | 0.0231 |
| q·Rg max | 1.3 | 1.3 | 1.3 | 1.3 | 1.3 |
| Coeff. correln (R2) | 0.98 | 0.99 | 0.96 | 0.98 | 0.86 |
| P(r) analysis | |||||
| I(0) (cm−1) | 0.00930 (4) | 0.01020 (5) | 0.00480 (1) | 0.00352 (7) | 0.00110 (5) |
| Rg (Å) | 18.7 (1) | 19.7 (1) | 24.5 (6) | 25.1 (5) | 20.1 (8) |
| Dmax (Å) | 57 | 60 | 77.9 | 78.2 | 60.1 |
| q range (Å−1) | 0.011–0.354 | 0.011–0.354 | 0.016–0.345 | 0.0180–0.276 | 0.023- 0.232 |
| Quality estimate | 0.87 | 0.85 | 0.88 | 0.89 | 0.85 |
| Porod vol (Å3) | 35,600 | 36,700 | 72,900 | 74,000 | 38,400 |
| MW (kDa) | 21.4 (0.97) b | 22.0 (0.88) d | 43.7 (1.99) b | 44.4 (2.02) b | 23.0 (0.92) d |
| MW | 23.3 (1.06) c | 25.3 (1.01) e |
a From deconvolution of SAXS data from A3BCTD* dimer + dZ-oligo. Note similarities in parameters to A3BCTD* dimer in absence of dZ-oligo and to A3BCTD*/dZ-oligo. b From Porod Volume*0.6 (Da) (ratio to expected size of A3BCTD* of 22,000 Da). c From I(0) (70)) (Da) (ratio to expected size of A3BCTD* of 22,000 Da). d From Porod Volume*0.6 (Da) (ratio to expected size of A3BCTD*/dZ-oligo subunit of 25,000 Da). e From I(0) (70)) (Da) (ratio to expected size of A3BCTD*/dZ-oligo*25,000 Da).