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. 2021 Feb 12;13(2):288. doi: 10.3390/v13020288

Figure 4.

Figure 4

GST pulldown between SMURF2 WW domains and full length VP40. (A) GST pulldown assay using purified GST-SMURF2 WW domains 1, 2, and 3 with HEK293T cell extracts containing eVP40 WT (lanes 1, 4, and 7), eVP40 PPxY mutant (lanes 2, 5, and 8), or GST alone (lanes 3, 6, and 9). (B) GST pulldown assay using purified GST-SMURF2 WW domains 1, 2, and 3 with HEK293T cell extracts containing mVP40 WT (lanes 1, 4, and 7), mVP40 PPxY mutant (lanes 2, 5, and 8), or GST alone (lanes 3, 6, and 9). Input levels of eVP40, mVP40, GST, and actin are shown.