Extended Data Figure 5. Correlation between L505 labeled protein and γ-secretase activity.

(a) Structures of JC8, L505, L646, GY4 and L631. (b) WB analysis of 11Bt labeled proteins in the absence or presence of its parent compound, a substrate binding site inhibitor pep11 and imidazole GSM, E2012. Labeled proteins were captured and analyzed by WB for PS1-NTF (n=1). (c) Pearson’s correlation between γ-secretase activity (Aβ40, Aβ42 cleavage rates in Fig. 4d) and L505 labeled PS1-NTF (in Fig. 5c) in LOAD samples (n=10). Linear regression analysis was used to calculate R and p values.