Table 3.
Main dehydration reagents for tissue dehydration
| Type | Chemicals | Features | Clearing methods or cocktails |
|---|---|---|---|
| Hydrophilic reagents | DMSO | Dehydration also induces a degree of transparency | DMSO/Sorbitol, FOCM |
| Glycerol | Scale, CLARITY | ||
| Sucrose | Single treatment, CUBIC, UbasM | ||
| Fructose | SeeDB, FRUIT | ||
| Sorbitol | DMSO/Sorbitol | ||
| Xylitol | ClearSee | ||
| Iohexol | RIMS, Ce3D, SeeDB2 | ||
| Iodixanol | SWITCH | ||
| Water-miscible polar solvents | Ethanol | Dehydration induces tissue shrinkage | BABB, Ethanol-ECi |
| Methanol | iDISCO+, Adipo-Clear, CalAdipoClear | ||
| 1-propanol | FluoClearBABB, 2ECi | ||
| Tert-butanol | FluoClearBABB, uDISCO, PEGASOS | ||
| THF | 3DISCO, FDISCO, vDISCO |