Figure 4.

Treatment of HSV-1 with embelin protects Vero cells from cytotoxic damage. The morphology of Vero cells under different experimental conditions are shown. Images were taken through a Biostar inverted microscope (Reichert) following 48 h incubation at 37 °C and 5% CO_2. (A) Vero cells; (B) Vero + HSV-1; (C) Vero cells + HSV-1 treated with 54 μM of Embelin; (D) Vero cells + 2% DMSO. Figure 4A shows Vero cells only and the morphology of the cells demonstrates a healthy, intact monolayer. Image 4B shows the effect of infecting Vero cells with HSV-1. Figure 4C shows no change in morphology of the Vero cells infected with HSV-1 treated with 54 μM of Embelin treated HSV-1 48 hpi. Figure 4D shows the administration of 2% DMSO presenting no toxicity for the vehicle solution.