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Cytotoxicity of corosolic acid (CA) and DE16-R fraction on cell lines 3T3-L1, HepG2 and C2C12. The cells were seeded in 96-well plates and treated for 48H (HepG2) (A), 4 h (C2C12) (B) and 7 days (3T3-L1) (C). The cytotoxicity was evaluated by methyl thiazole tetrazolium (MTT) assay. Values are expressed as means ± SEM. ANOVA with post hoc Dunnett’s for multiple comparisons was performed. ****: p < 0.0001; ***: p < 0.001; **: p < 0.01; *: p < 0.05. n = 3.
