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. 2021 Feb 18;10(2):431. doi: 10.3390/cells10020431

Figure 5.

Figure 5

In KRASmut MIA PaCa-2 and PANC-1 cells, FR054 and the pan-RAS inhibitor BI-2852 present additive negative effects on cell proliferation and survival. (A,B) Cell viability of MIA-PaCa-2 and PANC-1, treated for 72 h with FR054 (350 µM for MIA-PaCa-2, 500 µM for PANC-1) and different doses of RAS inhibitor BI-2852 (RASi), was detected through MTT test. (C–G) MIA PaCa-2 and PANC-1 were treated with FR054 and 50 µM BI-2852 for 72 h. (C–E) Cell death was evaluated by viable count using Trypan Blue stain (C,E) and by using flow cytometric analysis of annexin V-FITC and propidium iodide staining (D,F). Representative profiles of the flow cytometric analysis are shown on the right of the panels (D,F). (G) The protein expression was analyzed through Western blotting. Actin was used for signal normalization. All data represent the mean ± SEM of at least three independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 (One-way ANOVA), untreated vs. treated where not specifically indicated.