Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Homo sapiens) |
BLM | UniProt | P54132 | BLM_HUMAN |
| Gene (Homo sapiens) |
RECQL | UniProt | P46063 | RECQ1_HUMAN |
| Gene (Homo sapiens) |
WRN | UniProt | Q14191 | WRN_HUMAN |
| Gene (Homo sapiens) |
RECQL5 | UniProt | O94762 | RECQ5_HUMAN |
| Gene (Escherichia coli) |
uvrD | UniProt | P03018 | UVRD_ECOLI |
| Strain (Escherichia coli) |
BL21(DE3) | New England Biolabs | C2527I | Competent Cells |
| Sequence-based reagent | ssDNA-15mer | This paper | CGTACCCGATGTGTT | |
| Sequence-based reagent | ssDNA-20mer | This paper | CGTACCCGATGTGTTCGTTC | |
| Sequence-based reagent | Forked-50mer: FORK A |
This paper |
XGAACGAACACATCGGGTACG
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTT X = Black Hole Quencher 2 or none |
|
| Sequence-based reagent | Forked-50mer: FORK B |
This paper |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
CGTACCCGATGTGTTCGTTCY Y = Tetramethylrhodamine or none |
|
| Recombinant DNA reagent | pET-17b | Novagen Merck Millipore |
69663 | |
| Recombinant DNA reagent | pNIC28-Bsa4 | Addgene | 26103 | |
| Chemical compound, drug | ML216 | Merck KGaA Caymen Chemical |
SML0661 15186 |
|
| Commercial assay or kit | PiColorLock Gold Phosphate Detection System |
Novus Biologicals | 303–0030 | |
| Commercial assay or kit | DNA Unwinding Assay Kit |
Inspiralis | DUKSR001 | |
| Software, algorithm | BUSTER | Global Phasing | RRID:SCR_015653 | |
| Software, algorithm | CCP4 | CCP4 | RRID:SCR_007255 | |
| Software, algorithm | Coot | Coot | RRID:SCR_014222 | |
| Software, algorithm | Fiji | Fiji | RRID:SCR_002285 | |
| Software, algorithm | Phaser | Phaser | RRID:SCR_014219 | |
| Software, algorithm | PHENIX | PHENIX | RRID:SCR_014224S | |
| Software, algorithm | Prism | GraphPad | RRID:SCR_002798 | |
| Software, algorithm | XDS | XDS | RRID:SCR_015652 |