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. 2021 Mar 1;10:e63333. doi: 10.7554/eLife.63333

Figure 6. The homeostatic effects of tamoxifen are dependent on estrogen receptor alpha (ERα) in the hypothalamus and preoptic area (hypothalamus-POA).

Figure 6.

(A) Hourly average of core body temperature in Esr1 cKO female mice over 2 days before and 3 weeks after injections (dotted line). (B) Average core body temperature from mice shown in panel (A) highlighting per animal averages in light (7:00 to 19:00), dark (19:00 to 7:00), and total 24 hr periods (n = 8, treatment: F1,14=1.395, p=0.7144). (C) Heat loss index (HLI) calculated from continuous measurements of core and tail temperature. (D) Average HLI from mice shown in panel (C) (n = 7–8, treatment: F1,13=3.109, p=0.1013). (E) Thermographic images of interscapular skin above brown adipose tissues (BAT) depots in Esr1 cKO female mice injected with either oil or tamoxifen. (F) Quantification of temperature of skin above intrascapular BAT depots (n = 3, t4 = 0.264, p=0.8048). (G) Hourly average of movement over 2 days before and after injections (dotted line). (H) Average movement from mice shown in panel (H) (n = 8, treatment: F1,14=2.638, p=0.1266). (I) Representative micro-computed tomography (microCT) images showing bone density of the distal metaphysis and midshaft diaphysis of femurs from tamoxifen or oil treated Esr1 cKO female mice. (J) Trabecular bone volume fraction (BV/TV, t10 = 0.8340, p=0.4237), trabecular numbers (Tb.N, t10 = 3.349, p=0.0074), trabecular thickness (Tb.Th, t10 = 0.2668, p=0.7951), and trabecular separation (Tb.Sp, t10 = 1.924, p=0.0832) in distal metaphysis of femurs (n = 6). (K) Bone volume fraction (Ct.BV/TV, t10 = 0.5963, p=0.5643) and cortical thickness (Ct.Th, t10 = 0.9720, p=0.354) in diaphysis of femurs (n = 6). Line shading and error bars represent standard error of the mean (SEM). ns, non-significant; **, p<0.01 for Sidak's multiple comparisons test (B, D, and H) or two-tailed t-test (F, J, and K).

Figure 6—source data 1. Source data for Figure 6, panel a.
Hourly average of core body temperature in Esr1 cKO female mice over 2 days before and 3 weeks after injections. Each column is an independent mouse.
Figure 6—source data 2. Source data for Figure 6, panel b.
Average core body temperature from Esr1 cKO mice in light, dark, and total 24 hr periods. Each column is an independent mouse.
Figure 6—source data 3. Source data for Figure 6, panel c.
Hourly average of heat loss index (HLI) calculated from continuous measurements of core and tail temperature in Esr1 cKO female mice treated with oil or Tmx. Each column is an independent mouse.
Figure 6—source data 4. Source data for Figure 6, panel d.
Average heat loss index (HLI) in the light, dark, and total 24 hr periods for Esr1 cKO female mice treated with oil or Tmx. Each column is an independent mouse.
Figure 6—source data 5. Source data for Figure 6, panel f.
Quantification of temperature of skin above intrascapular brown adipose tissue (BAT) depots in Esr1 cKO female mice treated with oil or Tmx. Each cell is an independent mouse.
Figure 6—source data 6. Source data for Figure 6, panel g.
Hourly average of movement over 2 days before and 3 weeks after injections of oil or tamoxifen in Esr1 cKO female mice. Each column is an independent mouse.
Figure 6—source data 7. Source data for Figure 6, panel h.
Average movement in light, dark, and 24 hr periods over 2 days before and 3 weeks after injections of oil or tamoxifen in Esr1 cKO female mice. Each column is an independent mouse.