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. 2020 Feb 27;106(3):795–805. doi: 10.3324/haematol.2019.241232

Figure 3.

Figure 3.

Hematologic parameters and analysis of erythropoiesis in wild-type and Ncoa4-ko mice fed an iron-deficient diet. Ncoa4-ko and wild-type (wt) mice (of both genders) on a Sv129/J background were fed an iron-deficient diet for 6 months starting from the age of 3 months. Complete blood counts were determined periodically. (A) Values are shown for red blood cell count, hemoglobin levels, mean corpuscular volume and mean corpuscular hemoglobin. (B) Percentage of Ter119+ cells on alive cells and subpopulation composition (determined as described in Figure 1B) both in the bone marrow and in the spleen of 9-month old irondeficient wt and Ncoa4-ko mice. The complete statistical analysis is reported in Online Supplementary Table S5. (D) Colony numbers generated from 104 bone marrow cells of 9-month old iron-deficient wt and Ncoa4-ko mice. Mean values of four or five animals per genotype are shown. Error bars indicate the standard error. Asterisks refer to statistically significant differences between age-matched wt and Ncoa4-ko mice. *P<0.05; **P<0.01; ***P<0.005. ID: iron-deficient; RBC: red blood cells; Hb: hemoglobin; MCV: mean corpuscular volume; MCH: mean corpuscular hemoglobin; BM: bone marrow, SP: spleen; CFU-GM: colony-forming unit-granulocyte/ monocyte; BFU-E: burst-forming unit-erythroid.