Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Danio rerio) | Tg(7xTCF-Xla.Siam:GFP) | ZIRC | ZFIN ID:ZBD-ALT-110113–1 | |
| Recombinant DNA reagent | pCMV_BE4-gam (plasmid) |
Addgene | Addgene:#100806 RRID:Addgene_100806 |
|
| Recombinant DNA reagent | pCMV_ancBE4max (plasmid) |
Addgene | Addgene:#112094 RRID:Addgene_112094 |
|
| Recombinant DNA reagent | pCS2+_ancBE4max-SpymacCas9 (plasmid) |
This paper | See Materials and methods | |
| Commercial assay or kit | NEBuilder HiFi DNA Assembly Cloning Kit | New England Biolabs | Catalog# E5520S | |
| Commercial assay or kit | mMESSAGE mMACHINE T7 Ultra kit | Ambion | Catalog# AM1345 | |
| Commercial assay or kit | mMESSAGE mMACHINE Sp6 kit | Ambion | Catalog# AM1340 | |
| Commercial assay or kit | PCR clean-up gel extraction kit | Macherey-Nagel | Catalog# 740609.50 | |
| Peptide, recombinant protein | Phusion high-fidelity DNA polymerase | ThermoFisher | Catalog# F-530XL | |
| Software, algorithm | SequenceParser.py | This paper | See Source code 1 |