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. 2021 Feb 19;10(2):228. doi: 10.3390/pathogens10020228

Figure 2.

Figure 2

R. parkeri proliferates inside both endothelial cells (EA.hy926) and human derived macrophage cells (THP-1). (A,B) EA.hy926 cells and PMA-differentiated THP-1 cells were infected with R. parkeri st. Portsmouth (MOI = 2.5) and genomic DNA was extracted at each time point post-infection. Increase in growth is represented by the ratio of R. parkeri st. Portsmouth sca1 to the host cell actin gene determined by quantitative PCR (qPCR). A logistic regression test was used to measure significance (p < 0.05) in growth over time in both mammalian cell lines. Immunofluorescence microscopy demonstrated growth in EA.hy926 cells on days 1 and 4 post-infection (C) and in PMA-differentiated THP-1 cells on days 1 and 3 post-infection (D). Cells were stained with the following antibodies: DAPI (blue) to stain host cell nuclei, anti-Rickettsia antibody (RcPFA) followed by Alexa Fluor 488 (green) to stain R. parkeri st. Portsmouth, and Phalloidin (red) to stain actin. Scale bar = 10 μm.