Table 1. Expression of Selected Genes in the COX/PGE2/E-Prostanoid Receptor Pathway in the Aorta.
Relative mRNA expression of selected genes in PGE2 biogenesis including cyclooxygenase-1 (Cox-1), cyclooxygenase-2 (Cox-2); and prostaglandin E synthase (Ptges) 1-3 and PGE2 degradation (15-hydroxyprostaglandin dehydrogenase, Hpgd) as well as E-prostanoid receptors (EP1-4, also known as prostaglandin E2 receptor, Ptger1-4) were determined by quantitative real-time RT-PCR from aorta of the indicated mouse strains at the end of the 4-week study. Two-way ANOVA was performed with ΔCt values to test the effects of genotype (pgenotype) and diet (pdiet).
| Target Gene |
Two-way ANOVA | Ctrl. | NT Chow | NT HSD | S-P467L Chow | S-P467L HSD | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Pgenotype | Pdiet | Pinteraction | ΔCt | Mean (Lower-Upper) |
n | Mean (Lower-Upper) |
n | Mean (Lower-Upper) |
n | Mean (Lower-Upper) |
n | |
| Cox-1 | 0.09 | 0.73 | 0.80 | 0.7 | 1.00 (0.89-1.12) | 5 | 1.01 (0.93-1.10) | 6 | 0.79 (0.71-0.89) | 8 | 0.86 (0.76-0.96) | 8 |
| Cox-2 | 0.037 | 0.53 | 0.95 | 3.7 | 1.00 (0.81-1.23) | 5 | 1.10 (0.94-1.30) | 6 | 0.71 (0.62-0.82) | 8 | 0.81 (0.74-0.89) | 8 |
| Ptges-1 | 0.09 | 0.001 | 0.27 | 2.6 | 1.00 (0.98-1.02) | 4 | 0.59* (0.54-0.64) | 5 | 1.08 (0.98-1.19) | 8 | 0.81 (0.72-0.91) | 8 |
| Ptges-2 | 0.49 | 0.36 | 0.54 | 1.8 | 1.00 (0.95-1.05) | 5 | 0.88 (0.82-0.94) | 6 | 0.99 (0.92-1.07) | 8 | 0.97 (0.88-1.07) | 8 |
| Ptges-3 | 0.22 | 0.19 | 0.85 | 0.4 | 1.00 (0.90-1.11) | 5 | 0.84 (0.76-0.94) | 6 | 0.85 (0.79-0.92) | 8 | 0.75 (0.66-0.86) | 8 |
| Hpgd | 0.15 | 0.38 | 0.78 | 3.7 | 1.00 (0.78-1.29) | 5 | 1.20 (1.05-1.37) | 6 | 0.83 (0.75-0.92) | 8 | 0.93 (0.80-1.07) | 8 |
| EP1 | <0.001 | 0.96 | 0.94 | 10.8 | 1.00 (0.94-1.06) | 5 | 0.99 (0.83-1.19) | 4 | 1.63 (1.41-1.88) | 7 | 1.62 (1.39-1.87) | 7 |
| EP2 | 0.007 | 0.12 | 0.65 | 12.9 | 1.00 (0.93-1.08) | 5 | 0.68 (0.61-0.77) | 4 | 1.59 (1.38-1.83) | 7 | 1.30 (1.04-1.63) | 7 |
| EP3 | 0.03 | 0.51 | 0.75 | 7.1 | 1.00 (0.81-1.24) | 5 | 0.77 (0.68-0.87) | 4 | 1.62 (1.27-2.06) | 7 | 1.49 (1.11-1.98) | 7 |
| EP4 | 0.16 | 0.57 | 0.64 | 6.8 | 1.00 (0.89-1.12) | 5 | 1.01 (0.83-1.24) | 4 | 1.43 (1.22-1.67) | 7 | 1.41 (0.97-1.41) | 7 |
p<0.05, HSD compared to Chow Diet, Tukey’s multiple comparisons test, two-way ANOVA. ΔCt indicates the average of ΔCt values in the NT Chow Diet group. Group fold change relative to NT chow were calculated as 2^(−ΔΔCt) using the Livak method. Data are presented as mean values with upper and lower SEM in the parentheses. One outlier in NT Chow and one in NT HSD group were excluded from Ptges-1 expression based on Grubb’s test.