VOLUME 296 (2021) ARTICLE NUMBER 100023
In the current version of the manuscript it is stated that prey and bait vectors are linearized with EcoRI/BamHI for library insertion. However, the correct insertion is via NdeI/XhoI digest of the pGAD-HA prey plasmid and NdeI/NotI digestion of the pGBKT7 bait vector. To generate the prey strain, the 5 different sized CRAF fragments and MDM2 and RGL3 fragment libraries were cotransformed with linearized (NdeI/XhoI) pGAD-HA plasmids to generate pGAD-CRAF fragments in Y187 strain and Y2HGold-pGBKT7-KRAS (G12V) strain by homologous recombination (52, 53). The same procedures were followed for all other libraries. For the reverse assays, fragments were cloned into NdeI/NotI linearized pGBKT7 vector.