MEDICAL SCIENCES Correction for “PIWIL1 promotes gastric cancer via a piRNA-independent mechanism,” by Shuo Shi, Zhen-Zhen Yang, Sanhong Liu, Fan Yang, and Haifan Lin, which was first published August 26, 2020; 10.1073/pnas.2008724117 (Proc. Natl. Acad. Sci. U.S.A. 117, 22390–22401).
The authors note that Fig. 5 appeared incorrectly. In “panel A, the positions of the blue and red arrows were down-shifted by one band in the gel image. This error occurred when we were changing the initial two black arrows to blue and red arrows.” The corrected figure and its legend appear below.
Fig. 5.
PIWIL1 cooperates with UPF1 to negatively regulate PIWIL1-bound RNAs without piRNA. (A) A protein gel of PIWIL1-coimmunoprecipitation identifying PIWIL1 (blue arrow) and UPF1 (red arrow). (B–C) Western blots showing reciprocal coimmunoprecipitated between PIWIL1 and UPF1 (total and phosphorylated form, p-UPF1), between PIWIL1 and UPF2, and between. PIWIL1 and UPF1, p-UPF1, UPF2, and SMG1. (D) PIWIL1 (green) and DCP1A (red) in WT and PIWIL1KO SNU-1 cells. (E) PIWIL1 (green) colocalized with UPF1 (fuchsia) in the P body (red) in SNU-1 cells. (F) Venn diagram of PIWIL1/UPF1-bound RNAs, and PIWIL1-negatively regulated RNAs, P < 0.05, fold change ≥1.5. (G) Volcano plot of PIWIL1- and UPF1-negatively regulated direct targets in SNU-1 cells. Dotted lines represent 1.5-fold change in expression (vertical) and P < 0.01 cutoff (horizontal). (H) qRIP-PCR confirmed that the six indicated genes are bound by UPF1. Mean ± SD; n = 3. (I) KEGG pathway analysis of 203 RNAs targeted by both PIWIL1 and UPF1. (J) Docking model of PIWIL1 (yellow), UPF1 (green), and RNA (orange). Schematic of full-length PIWIL1 and UPF1-interacting domain mutants-PIWIL1. (K) Coimmunoprecipitation mapping of the UPF1-interacting domain of PIWIL1. pcDNA-Flag: empty vector. (L) Western blotting of coimmunoprecipitation showing that Flag-piRNA-BM-PIWIL1 interacts with UPF1 as strongly as WT PIWIL1 (Flag-PIWIL1-FL). (M) qRT-PCR showing that the up-regulation of PIWIL1-UPF1 cotargeted mRNAs in PIWIL1-KO cells can be rescued by WT-PIWIL1 but not ΔNΔC-PIWIL1. Mean ± SD; n = 3. (N) Transwell assays showing that the inhibition of cell migration in PIWIL1-KO cells can be rescued by WT-PIWIL1 but not ΔNΔC-PIWIL1; n = 3. (O) Bar graph shows the numbers of migrated cells of each view in the Transwell assay (n = 3). **P < 0.01, paired t test.
In addition, the authors note that “The SI Appendix published with Fig. 5 instead of Fig. S5. This mistake was made during the post-review reassembly of supplementary figures, so it did not affect the review process.” The SI Appendix has been corrected online.