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. 2021 Feb 15;10:e59119. doi: 10.7554/eLife.59119

Figure 5. BBS3 interacts with the BBSome in cilia in a GTP-dependent manner.

Figure 5.

(A) Immunoblots of sucrose density gradient of ciliary extracts (CE) of BBS3::YFP and BBS3A73L::YFP cells probed with α-BBS1, α-BBS5, and α-YFP. (B) Immunoblots of α-YFP-captured proteins from CE of BBS3A73L::YFP and BBS3::YFP cells probed with α-BBS1 and α-BBS5. (C) Immunoblots of sucrose density gradient of CE of BBS3::YFP cells, in the presence of GTPγS or GDP, probed with α-BBS1, α-BBS5, and α-GFP. (D) Immunoblots of α-YFP-captured proteins from CE of BBS3::YFP cells, in the presence of GTPγS, GDP, or neither, probed with α-BBS1 and α-BBS5. For panels B and D, input was quantified with α-YFP by immunoblotting.