Extended Data Fig. 4. Manipulation of glycolysis, or carbon or nitrogen supply, has no effect on D. discoideum aggregation.

a, Schematic showing targets of the glycolytic inhibitors 2-deoxyglucose (2-DG) and koningic acid (KA). b, c, Starved D. discoideum cultured with 2-DG (n = 3) (b) or KA (n = 3) (c). d–g, Starving D. discoideum were cultured with non-essential amino acids (NEAA) (n = 3) (d), glucose (n = 3) (e), ammonia NH₃ (n = 3) (f) or a combination of glucose and NH₄Cl (n = 3) (g) for 8 h.