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. 2021 Feb 19;11(9):4137–4154. doi: 10.7150/thno.54066

Figure 4.

Figure 4

In vitro cytotoxicity of free cabazitaxel and pLAn-SS-CTX NPs (n = 15 or 50) against human cancer cell lines including A) A549, B) resistant A549/PTX , C) HeLa, D) resistant HeLa/PTX cells. E) P-gp levels in A549, A549/PTX, HeLa, and HeLa/PTX cells were assessed by western blot. F) Western blot analysis of apoptosis- and microtubule-associated proteins in DU145 and HeLa/PTX cells following the incubation with free cabazitaxel and pLAn-SS-CTX NPs (at a cabazitaxel-equivalent concentration, 10 nM for DU145 cells and 25 nM for HeLa/PTX cells). G) Representative fluorescence microscopy images suggest increased tubulin acetylation using the staining with acetyl-α-tubulin antibody. The cells were treated with free cabazitaxel and pLAn-SS-CTX NPs. H) Anti-proliferative effect of drugs (2 nM for DU145 cells and 6 nM for HeLa/PTX cells, cabazitaxel-equivalent concentration) determined by the Click-iT EdU assay. I) Acridine orange/ethidium bromide (AO/EB) assay for evaluation of cell apoptosis. Cells were treated with free cabazitaxel and pLAn-SS-CTX NPs for 48 h. The cell apoptotic ratio was defined as the ratio of apoptotic cells (brightly orange cells) to total cells (green cells). The data are presented as the means ± SD.