Extended Data Table 4.

Abundance of human ACE2 and TMPRSS2 transcripts detected in SARS-CoV-2 infected LoM.

Group	Mouse	Human donor	GAPDH (TPM)	ACE2 (TPM)	TMPRSS2 (TPM)
Day 2	1	A31	517.41	1.79	33.54
	41	I32	432.19	4.63	41.96
Day 6	31	R30	654.63	2.67	78.64
	32	A31	784.39	0.73	21.62
	33	I32	481.94	1.56	41.07
Day 14	37	I32	419.55	2.02	38.94
	39	F32	492.57	1.90	61.95
	42	A31	351.54	1.54	43.50
Naive	43	A31	2384.62	0.19	15.35
	44	I32	363.42	0.82	38.95
	45	I32	359.21	0.67	38.19
	46	I32	339.77	0.89	32.93

Shown are the abundance of human GAPDH, ACE2, and TMPRSS2 transcripts sequenced in the human lung tissue collected from naïve (n=4) and SARS-CoV-2 infected (n=8) LoM. Median ACE2 and TMPRSS2 expression in human lung tissue of naïve mice was 0.75 TPM and 35.6 TPM respectively, which is comparable to the median expression of ACE2 (1.01 TPM) and TMPRSS2 (43.2 TPM) observed in human lung tissue profiled by the GTEx project (https://gtexportal.org/home/; data retrieved 10/20/2020 and 11/3/2020). No effect of human donor on ACE2 expression (F = 2.06259641; p=0.1756 via two-sided ANOVA) was observed. Approximately the same amount of variation was observed within a human donor (mean variance within donors = 4.82) as across all donors (variance = 4.47). n=number of biologically independent lung tissues analyzed.