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. 2021 Feb 18;40(11):1974–1987. doi: 10.1038/s41388-021-01682-z

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© The Author(s) 2021, corrected publication 2021

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Fig. 3 — a Immunohistochemistry (IHC) of CHRNA7 in 104 human ESCC tissues and their paired adjacent normal tissues (left panel). Bar charts showing the percentage of cases with different levels of CHRNA7 in tumors and their paired adjacent normal tissues (right panel). b Kaplan–Meier curves compared the overall survival in ESCC patients with high and low protein levels of CHRNA7. c RT-qPCR (left panel) and immunoblot (right panel) analysis of CHRNA7 in KYSE270 and TE1 cells treated with nicotine or vehicle. GAPDH was used as an internal control. d Flow cytometry analysis of ALDH-positive population in TE1 cells transfected with shCHRNA7 #1 or shCtrl were treated with or without nicotine (top panel). Histograms showing the proportion of ALDH-positive cells (bottom panel). e Flow cytometry analysis of CD44-positive population in TE1 cells transfected with shCHRNA7 #1 or shCtrl were treated with or without nicotine (top panel). Histograms showing the proportion of CD44-positive cells (bottom panel). Data are shown as the means of three independent experiments or representative data. Error bars indicate SD. **P < 0.01, ***P < 0.001 by Wilcoxon matched-pair signed-rank test or one-way ANOVA with post hoc intergroup comparisons.