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. 2021 Feb 24;40(11):2112–2129. doi: 10.1038/s41388-021-01679-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Genomic alterations of USP13 across human cancers determined by cBioportal analysis of TCGA data. B Scatter dot plot analysis of USP13 mRNA expression against USP13 copy number alterations in cervical cancer determined by cBioportal analysis of TCGA data. Correlation was determined using Spearman’s analysis. C RT-qPCR analysis of USP13 mRNA expression in normal human keratinocytes (NHKs), HPV- C33A cells, HPV16 + SiHa and CaSKi cells and HPV18 + SW756 and HeLa cells. mRNA expression was normalized against U6 mRNA levels. D Representative western blot of USP13 expression in NHKs, C33A cells, SiHa, CaSKi, SW756 and HeLa cells. GAPDH served as a loading control. Quantification of the protein band intensities from four biological, independent repeats are shown on the right. E Representative immunohistochemical (IHC) staining of USP13 expression in cervical cancer tissues and normal cervical epithelium from a tissue microarray (TMA). Scale bars, 100 μm. Scatter dot plot analysis of USP13 expression from a larger cohort of cervical cancer cases (n = 41) and normal cervical epithelium (n = 9) is shown on the right. F Scatter dot plot of RT-qPCR analysis of USP13 mRNA expression from a panel of cervical cytology samples representing CIN lesions of increasing grade. Five samples from each clinical grade (negative (Neg) and CIN I-III) were analysed and mRNA levels were normalized to the negative samples. Samples were normalized against U6 mRNA levels. G Representative western blot of cervical cytology samples of CIN lesions of increasing grade analysed for USP13 protein expression. GAPDH served as a loading control. Scatter dot plot analysis of a larger cohort of samples (n = 15 for each grade) is shown on the right. Bars represent the mean ± standard deviation from at least three biological repeats unless otherwise stated. *p < 0.05; **p < 0.01; ***p < 0.001 (Student’s t-test).