Anti–PD-1 Monotherapy for Advanced NSCLC Patients with Older Age or Those with Poor Performance Status

Abstract

Purpose

Anti-programmed death 1 (PD-1) antibodies have emerged as frontline treatments for patients with advanced non-small cell lung cancer (NSCLC) on the basis of global Phase III trials. However, current data regarding responses to anti–PD-1 therapy in older patients with NSCLC or those with poor performance status (PS) are limited. Therefore, we examined the therapeutic effect of anti PD-1 antibody in these patients.

Patients

We retrospectively examined consecutive patients treated with anti–PD-1 monotherapy (pembrolizumab or nivolumab) from January 2016 to September 2018.

Results

We enrolled 125 patients (median age, 60 years), 80.8% of whom were men. Patients aged ≥75 years were considered older patients (n = 15), and those with PS 2–3 were regarded as having poor PS (n = 11). The objective response and disease control rates were 15.4% and 46.2%, respectively, in older patients and 9.1% and 27.3%, respectively, in those with poor PS. Progression-free survival (PFS) and overall survival (OS) did not significantly differ between older and younger patients. However, poor PS was significantly associated with poor survival. High programmed death ligand 1 (PD-L1) expression in tumor specimens (≥50%) was associated with favorable survival in the entire cohort as well as patients with poor PS. Safety analyses demonstrated no significant difference in the occurrence of any adverse event, including grade ≥3 adverse events, between patients with poor PS or older age and the remaining patients.

Conclusion

Anti–PD-1 therapy had similar efficacy in older and younger patients with NSCLC, whereas survival was significantly worse in patients with poor PS. However, immune checkpoint inhibitors may be considered for patients with poor PS harboring positive PD-L1 expression.

Introduction

Lung cancer is one of the most fatal malignancies globally, and non-small cell lung cancer accounts for approximately 85% of all lung cancers.¹ Chemotherapy has been required to prolong the survival of patients with unresectable or recurrent lung cancer. Over the past decade, the therapeutic management of NSCLC transitioned from conventional therapy to molecular targeted therapy based on the mutation status of oncogenic driver genes such as epidermal growth factor receptor (EGFR) or anaplastic lymphoma kinase.^2,3 However, the prognosis of patients who do not harbor specific oncogenic gene mutations remained poor even after the discovery of these therapeutic agents.

Immune checkpoint inhibitors (ICIs), such as programmed death 1/programmed death ligand 1 (PD-1/PD-L1) inhibitors, conferred survival advantages compared with standard chemotherapies, and they have become important treatment options for NSCLC.^4–6 Nivolumab, a monoclonal antibody against PD-1, was first approved for the treatment of patients with metastatic NSCLC who experienced disease progression during or following platinum-based chemotherapy. Subsequently, pembrolizumab, a monoclonal antibody against PD-1, was approved as monotherapy for both NSCLC in the first-line setting and metastatic NSCLC following disease progression during or following platinum-based chemotherapy. Nosaki et al reported that pembrolizumab provided favorable survival outcomes compared with chemotherapy even in elderly patients via a pooled analysis of three prospective clinical trials.⁷ However, the number of older patients (≥75 years) was small or unknown, and the performance status (PS) of the enrolled patients was good (0 or 1) in these clinical studies.

Although previous retrospective studies have investigated the efficacy or survival benefit of anti–PD-1 therapy in older patients or those with PS 2–4,^8,9 it is important to accumulate more clinical data on these cohorts and identify populations that derive clinical benefit from anti–PD-1 therapy. Thus, we examined the therapeutic benefit and safety profile of PD-1 therapy in older patients or those with poor PS.

Patients and Methods

Patients

We retrospectively examined 125 consecutive patients who received anti–PD-1 monotherapy (pembrolizumab or nivolumab) from January 2016 to September 2018 at the Department of Thoracic Oncology, National Hospital Organization Kyushu Cancer Center. These patients were diagnosed with unresectable advanced-stage NSCLC or recurrent disease after curative pulmonary resection. The following clinicopathological characteristics were investigated: age, sex, smoking status, Eastern Cooperative Oncology Group (ECOG) PS, line of treatment, histological type, oncogenic mutation status (EGFR), clinical stage, type of anti PD-1 therapy, and PD-L1 expression in tumor specimens according to immunohistochemistry (monoclonal antibody, 22C3, Dako, Carpinteria, CA, USA). The clinical stage of each patient was defined according to the criteria of the eighth edition of the TNM classification of the International Association for the Study of Lung Cancer.¹⁰

Patients received nivolumab intravenously at a dose of 3 mg/kg every 2–3 weeks or pembrolizumab intravenously at a fixed dose of 200 mg every 3–4 weeks in the clinical setting. Treatment continued until disease progression, treatment-related adverse events (trAEs), or death. Treatment response was assessed using the Response Evaluation Criteria in Solid Tumors every 6–10 weeks.¹¹ The toxicities of anti–PD-1 therapy were evaluated according to Common Terminology Criteria for Adverse Events version 4.0. TrAEs were defined as all adverse events (AEs) considered associated with ICIs, including immune-related adverse events (irAEs) and non-irAEs. Events caused by other etiologies (infections or other organic diseases) were excluded.^12,13 Of these AEs, treatment-related pneumonitis was diagnosed on a clinical and radiological basis. Other common causes of pulmonary decompensation, including progression of malignancy, chronic obstructive pulmonary disease, and infection, were excluded. Clinical information and follow‐up data were obtained from patients’ medical records. Written informed consent was obtained from each patient before inclusion in this study. Our study was conducted in accordance with the Declaration of Helsinki, and was approved by our institutional review board (National Hospital Organization Kyushu Cancer Center, IRB No: 2019–56).

Statistical Analysis

We performed all statistical analyses using JMP software version 14 (SAS Institute Inc., Cary, NC, USA). Continuous variables were expressed as the mean ± SD, and categorical variables were expressed as numbers. Differences between two groups for response rate or AEs were assessed using a two-sided Fisher’s exact test. The objective response rate (ORR) was defined as the proportion of patients with a partial or complete response to anticancer therapy. The disease control rate (DCR) was a composite of ORR and stable disease. Progression-free survival (PFS) was defined as the time from treatment initiation to the date of disease progression or death. Overall survival (OS) was defined as time from treatment initiation to death from any cause. Hazard ratios (HRs) and 95% confidence intervals (CIs) were estimated via the Cox proportional hazards model. Multivariate analyses were used to estimate the HRs for independent prognostic values via Cox proportional hazards regression models with the backward elimination method. All factors assessed in the univariate analysis were included in the multivariate analysis. P < 0.05 was regarded as statistically significant.

Results

Patient Background

The baseline characteristics of the 125 included patients are presented in Table 1. Of these patients, the median age was 60 years (range, 31–85) years, and 15 patients (12.0%) were at least 75 years old (older group). In our cohort, the proportions of patients treated with nivolumab and pembrolizumab were approximately equal. One hundred fourteen patients had a PS of 0 or 1 (good PS, 71 and 43 patients, respectively), whereas 11 patients had a PS of 2 or 3 (poor PS, 9 and 2 patients, respectively). The major histological type was adenocarcinoma (61.6%), and 10 patients (8.0%) harboring EGFR mutations received anti–PD-1 therapy, and 54 patients received anti–PD-1 therapy in the first- or second-line setting and 71 patients were treated in the third or later lines.

Table 1.

Clinical Characteristics of the Enrolled 125 Patients

Factors		Number of Patients =125
Age	Median (range)	60 (31–85)
Sex	Male	101
	Female	24
Smoking status	Never	19
	Ever	106
Performance status (PS)	0 −1	114
	2 −3	11
Histological type	Ad	77
	Sq or other type	48
EGFR	Wild-type	102
	Mutant	10
	Unknown	13
Anti PD-1 antibody	Nivolumab	63
	Pembrolizumab	62
Stage	III or IV	108
	Recurrent after surgery	17
Treatment line	≤2nd	54
	>3rd	71
PD-L1 expression	<1%	21
	1–49%	27
	≥ 50%	45
	Unknown	32
Treatment after anti-PD-1 therapy	Systemic	51
	Anti-PD-1 therapy	3
	Anti-PD-L1 therapy	1
	Cytotoxic therapy	47
	Local	3
	BSC (including palliative radiation therapy)	71

Abbreviations: EGFR, epidermal growth factor receptor; PD-1, programmed death 1; PD-L1, programmed death ligand 1; Ad, adenocarcinoma; Sq, squamous cell lung cancer; BSC, best supportive care.

Regarding PD-L1 expression in tumor tissues, 48 patients (36.8%) exhibited no or moderate expression (1%–49%), and 45 patients had high expression (≥50%). The PD-L1 data of 32 patients could not be obtained.

Efficacy

The median follow-up time was 13.8 months (range, 0.1–43.9 months). In the entire cohort, ORR and DCR were 26.4% and 49.6%, respectively. As presented in Figure 1, there were no significant differences in the ORR or DCR between older and younger age or good and poor PS (age: P = 0.232, and P = 0.418; PS: P = 0.285, and P = 0.205, respectively).

Figure 1.

(A) Therapeutic response in older patients treated with immune checkpoint inhibitors. (B) Therapeutic response in patients with poor performance status treated with immune checkpoint inhibitors.

Abbreviations: ORR, objective response rate; DCR, disease control rate.

Next, we performed survival analyses. As presented in Figure 2, survival did not differ between older and younger patients (PFS, HR = 1.15 [95% CI = 0.63–2.12], P = 0.647; OS, HR = 1.06 [95% CI = 0.55–2.06], P = 0.858). Contrarily, PFS (HR = 2.00 [95% CI = 1.00–4.00], P = 0.045) and OS (HR = 2.95 [95% CI = 1.47–5.94], P = 0.0014) were significantly worse among patients with poor PS. Furthermore, we performed survival analyses according to tumor PD-L1 expression. In the entire cohort, high PD-L1 expression was associated with better survival (Figure 3A and B). In the poor PS group, positive PD-L1 expression was associated with better survival using cut-off values of both 1% and 50% (Figure 3C and D).

Figure 2.

Kaplan–Meier curves for progression-free survival (A) and overall survival (B) among older and younger patients treated with immune checkpoint inhibitors. Kaplan–Meier curves for progression–free survival (C) and overall survival (D) among patients with performance status (PS) 0–1 or 2–3.

Figure 3.

Overall survival following treatment with immune checkpoint inhibitors according to tumor programmed death ligand 1 (PD-L1) expression (the cut-off values of PD-L1 expression were (A) 50% and (B) 1%, respectively), and subgroup analysis of patients with poor performance status according to tumor PD-L1 expression (the cut-off values of PD-L1 expression were (C) 50% and (D) 1%, respectively). Patients lacking PD-L1 data were excluded in these analyses.

Finally, we performed univariate and multivariate analyses to identify independent prognostic factors. In addition to PS and PD-L1 expression, the occurrence of trAEs and line of therapy were significantly associated with patient survival. In multivariate analysis, poor PS and later line of therapy (3rd-) were independent factors that predicted poor survival (Supplementary Table 1).

Safety

Table 2 presents anti–PD-1 trAEs. We compared the incidence of AEs between patients with poor PS or older age and the remaining patients. The rate of trAEs and that of severe trAEs (grade ≥3) did not significantly differ when comparing age or PS. Severe pneumonitis was identified in three patients (20.0%) of older age, and it was more frequent in this group than in the remaining patients (P = 0.0546), although this difference was not statistically significant.

Table 2.

Treatment-Related Adverse Events Among Patients Aged ≥75 Years or Those with Poor PS

Population
Events	Older (n=15)	Younger (n=110)	P-value	Poor PS (n=11)	Good PS (n=114)	P-value
Any events
All grades	11 (73.3%)	72 (65.5%)	0.7717	5 (45.5%)	78 (68.4%)	0.1794
Grade ≥3	5 (33.3%)	14 (12.7%)	0.0526	2 (18.2%)	17 (14.9%)	0.6736
Pneumonitis
All grades	4 (26.7%)	15 (13.6%)	0.2430	2 (18.2%)	17 (14.9%)	0.6736
Grade ≥3	3 (20.0%)	5 (4.6%)	0.0546	1 (9.1%)	7 (6.1%)	0.5321
Dermatitis
All grades	3 (20.0%)	33 (30.0%)	0.5512	1 (9.1%)	35 (30.7%)	0.1751
Grade ≥3	0 (0.0%)	1 (0.9%)	1.0000	0 (0.0%)	1 (0.9%)	1.0000
Thyroiditis
All grades	1 (6.7%)	2 (1.8%)	0.3208	0 (0.0%)	3 (2.6%)	1.0000
Grade ≥3	0 (0.0%)	0 (0.0%)	1.0000	0 (0.0%)	0 (0.0%)	1.0000
Colitis
All grades	2 (13.3%)	6 (5.5%)	0.2458	0 (0.0%)	8 (7.0%)	1.0000
Grade ≥3	0 (0.0%)	2 (1.8%)	1.0000	0 (0.0%)	2 (1.8%)	1.0000
Hepatitis
All grades	0 (0.0%)	5 (4.6%)	1.0000	0 (0.0%)	5 (4.4%)	1.0000
Grade ≥3	0 (0.0%)	1 (0.9%)	1.0000	0 (0.0%)	1 (0.9%)	1.0000
Adrenal failure
All grades	0 (0.0%)	3 (2.7)	1.0000	0 (0.0%)	3 (2.6%)	1.0000
Grade ≥3	0 (0.0%)	1 (0.9%)	1.0000	0 (0.0%)	1 (0.9%)	1.0000
Othersa
All grades	6 (40.0%)	37 (33.6%)	0.7728	4 (36.4%)	39 (34.2%)	1.0000
Grade ≥3	2 (13.3%)	6 (5.5%)	0.2458	1 (9.1%)	7 (6.1%)	0.5321

Note: ^aOthers: fatigue, fever, embolism, anorexia, pericarditis, mucositis, nausea, myalgia, arthralgia, and any outliers of laboratory test.

Abbreviation: PS, performance status.

Discussion

The present study investigated the efficacy and safety of anti–PD-1 therapy in patients with older age and/or poor PS. In this real-world retrospective study, anti–PD-1 therapy provided comparable efficacy in older patients, but survival was worse in patients with poor PS than in their counterparts with good PS. However, positive PD-L1 expression was associated with better survival in poor PS group. Regarding the safety profile, overall all-grade trAE rates did not differ between patients with poor PS or older age and the remaining patients.

Several meta-analyses of clinical trials suggested that the efficacy of anti–PD-1 therapies was similar in older and younger patients.^14–16 However, the older patients included in these trials were highly selected. In clinical practice, high numbers of older patients require systemic chemotherapy. Thus, it is extremely important to identify the efficacy and safety profiles of ICIs in older patients in the real-world setting. A multicenter retrospective study in Canada and France revealed no relationships between age as a categorical variable (<60, 65, 70, 75, and ≥75 years) and survival outcomes.¹⁷ Furthermore, the study revealed that the rate of immune-related AEs was statistically similar between older (≥70 years) and older patients. Corbaux and colleagues investigated survival analyses including 410 patients from three institutions who received ICIs for NSCLC and melanoma.¹⁸ They performed univariate and multivariate analyses to identify the prognostic factors of PFS and OS among patients treated with anti–PD-1 inhibitors and demonstrated that post-treatment survival outcomes did not differ by age. These previous findings are mostly consistent with the results of the present study. Conversely, Lichtenstein et al found that patients older than 80 years had significantly lower OS than their counterparts in a study of 245 patients with NSCLC who were treated with ICIs.¹⁹ However, this result might have been influenced by the higher proportion of male in the older group. These retrospective and real-world studies provided us insights into the critical management of older patients receiving ICIs.

Furthermore, PS is known as a significant prognostic factor for patients with malignant tumors. Several reports investigated the efficacy and safety of ICIs in patients with poor PS. Our study demonstrated that poor PS was associated with lower ORR and significantly worse survival than good PS. This result was consistent with the findings of several previous studies. Katsura et al compared treatment efficacy by PS status in patients who received nivolumab.²⁰ The study found that patients with PS 2–4 (poor PS group) had significantly shorter survival than those with PS 0–1 (good PS group), and survival in the poor PS group was similar to that in patients who received best supportive care (BSC). They also reported that the frequency of severe pneumonitis was significantly higher in the poor PS group than in the good PS group, which was similar to the present study. Naqash et al investigated clinical factors that were associated with survival after nivolumab treatment.²¹ They reported that age was not associated with the PFS and OS, but PS 2–4 was significantly associated with poor survival by multivariate analyses, which is consistent with our results. Furthermore, a meta-analysis of 19 studies that explored the prognostic impact of PS ≥2 on survival in patients NSCLC who received ICIs suggested that PS at the start of ICI treatment had prognostic significance, but the predictive value of PS 2 regarding the response to ICIs remained unclear.²²

These previous reports and the present study suggested that ICI treatment should not be recommended for patients with NSCLC and poor PS, but can we conclude that these patients are truly ineligible for ICI therapy? The results of our prior survival analyses indicated that PD-L1 expression in tumor specimens could be one of the keys for optimizing therapeutic intervention in populations with poor survival. Of note, treatment efficacy was identified even in patients with at least 1% PD-L1 expression in tumor specimens. PD-L1 expression is known as a predictive biomarker for clinical response to pembrolizumab or nivolumab.^23–25 Similar to our study, Jodai et al reported that patients with NSCLC and poor PS (≥2) and ≥50% PD-L1 expression were expected to benefit from anti–PD-1/PD-L1 therapy.²⁶ Based on these reports, ICI introduction may be considered for patients with positive PD-L1 expression, even if they have poor PS.

Our study had some limitations, including its retrospective, single-institutional nature. In addition, the older and poor PS populations were relatively small, which may have affected the results. Second, our cohort included men and adenocarcinoma patients predominantly. A previous report showed women were more likely to develop irAEs than men, and women with irAEs had significantly longer survival than those without irAEs. Thus, these population biases might have had a strong influence on our results. Finally, the present study did not compare outcomes in patients who received other cytotoxic chemotherapies or BSC. We would like to perform this research in the future to examine the benefits of ICIs in older or poor PS populations.

In conclusion, although age was not predictive of survival following ICI therapy in patients with NSCLC, patients with poor PS had a worse prognosis than those with good PS. However, it may be worth considering ICIs for patients with positive PD-L1 expression, even those with poor PS.

Funding Statement

This research did not receive grants from funding agencies.

Disclosure

The authors declare no conflicts of interest.