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. 2020 Oct 31;44(2):737–745. doi: 10.1007/s10753-020-01373-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Effects of extracellular acidosis on F-actin organization of DCs. Confocal microscopy analysis was performed on DCs to analyze the effects of extracellular acidosis on F-actin organization in the acidic microenvironment (a, c) or after removing it (b, d). a, b The 3-dimensional images from a representative experiment are shown. The magnification used is × 600. c, d Results are expressed as mean fluorescence intensity values and represent the arithmetic mean ± SD of three experiments. The value of the pH 7.3 group at the 1 h after acidosis treatment (c) and removing acidosis (d) was set to 1.0. Statistically significant difference was indicated by bars with asterisks (*p < 0.05; **p < 0.01).