Extended Data Figure 7. Tyraminergic inhibition of stress response depends on the DAF-2 insulin receptor.

a, Survival percentage of animals exposed to oxidative stress (3 h, 15 mM Fe²⁺) or heat (7 h, 35°C,). tdc-1; daf-2 and tyra-3; daf-2 double mutants are as resistant as daf-2 single mutant animals. Bars represent mean ± SEM, n = 3 (heat) and n=4 (oxidation), 80–100 animals per condition per experiment. One-way ANOVA followed by Holm-Sidak’s test for multiple comparisons compared to daf-2 mutants. b, Survival percentage of animals exposed to oxidative stress (3 h, 15 mM Fe^2+, top) or heat (7 h, 35°C, bottom) in the absence and presence of exogenous TA (10 mM). Detrimental effect of exogenous TA heat or oxidative stress resistance is not observed in daf-2 mutant animals. Bars represent the mean ± SEM, n = 3 (heat) and n=4 (oxidation). 80–100 animals per condition per experiment. Two-tailed Student’s t-test versus each strain without TA. This indicates that tyraminergic regulation of environmental stress response depends on daf-2.