Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Biological sample (human) | Peripheral blood mononuclear cells | Singapore Immunology Network, National Heart Centre, Singapore |
Frozen PBMCs from healthy participants, convalescent COVID-19 patients, non-COVID-19 patients with cardiovascular risk | |
| Antibody | Anti-mouse Ig, κ/negative control compensation particles set | BD Bioscience, San Jose, California, United States |
Cat# 552843 RRID: AB_10051478 |
To optimize fluorescence compensation settings for Flow Cytometry |
| Antibody | Hoechst 33342 Ready Flow Reagent | Invitrogen, Thermo Fisher Scientific, Waltham, Massachusetts, United States |
Cat# R37165 | Flow Cytometry (5:100) |
| Antibody | PE/cyanine7 anti-human CD31 antibody (mouse IgG1, κ, clone# WM59) | Biolegend, San Diego, California, United States |
Cat# 303118 RRID: AB_2247932 |
Flow Cytometry (4:100) |
| Antibody | APC anti-human CD133 antibody (mouse IgG1, κ, clone# clone 7) | Biolegend, San Diego, California, United States |
Cat# 372806 RRID: AB_2632882 |
Flow Cytometry (4:100) |
| Antibody | Human CX3CL1/fractalkine chemokine domain Alexa Fluor 488-conjugated antibody (monoclonal mouse IgG1 clone# 51637) | Research And Diagnostic Systems, Inc (R&D Systems, Inc), Minneapolis, Minnesota, United States |
Cat# IC365G-100UG RRID: AB_2885194 |
Flow Cytometry (6:100) |
| Antibody | Alexa Fluor 488 anti-human CD62P (P-selectin) antibody (mouse IgG1, κ, clone# AK4) | Biolegend, San Diego, California, United States |
Cat# 304916 RRID: AB_10719839 |
Flow Cytometry (5:100) |
| Antibody | PE anti-human CD45 antibody (mouse IgG1, κ, clone# 2D1) | Biolegend, San Diego, California, United States |
Cat# 368510 RRID: AB_2566370 |
Flow Cytometry (3:100) |
| Antibody | BV711 mouse anti-human CD54 (mouse BALB/c IgG1, κ, clone# HA58 (RUO)) | BD Bioscience, San Jose, California, United States |
Cat# 564078 RRID: AB_2738579 |
Flow Cytometry (5:100) |
| Commercial assay or kit | Cytokine/ chemokine/ growth factor 45-Plex Human ProcartaPlex Panel 1 | Invitrogen, Thermo Fisher Scientific, Waltham, Massachusetts, United States |
Cat# EPX450-12171-901 | Luminex assay |
| Software, algorithm | Bio-Plex Manager 6.1.1 software | Bio-Rad Laboratories, Hercules, California, United States |
Data analysis of multiplex assay | |
| Software, algorithm | GraphPad Prism, version 8.3.1 | GraphPad Software | Data analysis, statistics, and graphing | |
| Software, algorithm | FACSDiva software, version 8.0.1 | BD Bioscience, San Jose, California, United States |
Flow data acquisition | |
| Software, algorithm | FlowJo software, version 10.7.1 | BD Bioscience, San Jose, California, United States |
Flow analysis | |
| Software, algorithm | STRING (Search Tool for the Retrieval of Interacting Genes/Proteins) | ELIXIR Core Data Resources, Europe | Protein-Protein interaction networks and functional enrichment analysis | |
| Software, algorithm | Cellxgene | The Chan Zuckerberg Initiative Wilk et al dataset: https://cellxgene.cziscience.com/d/Single_cell_atlas_of_peripheral_immune_response_to_SARS_CoV_2_infection-25.cxg/ Schulte-Schrepping et al dataset: https://beta.fastgenomics.org/datasets/detail-dataset-952687f71ef34322a850553c4a24e82e#Cellxgene |
Analysis and visualization of scRNA-seq data |