Table 1:
Targeted Rickettsia screen of aquatic/semiaquatic invertebrates
| Aquatic/semiaquatic invertebrate group | Species | Location | Year | No. tested | No. positive |
|---|---|---|---|---|---|
| Ephemeroptera | Baetis muticus | Stirling, Scotland, UK | 2017 | 3 | 0 |
| Baetis rhodani | Stirling, Scotland, UK | 2017 | 3 | 0 | |
| Cloeon dipterum | Cheshire, UK | 2016 | 3 | 0 | |
| Ecdyonurus sp. 1 | Stirling, Scotland, UK | 2017 | 5 | 0 | |
| Ecdyonurus sp. 2 | Cheshire, UK | 2016 | 3 | 0 | |
| Ecdyonurus venosus | Cheshire, UK | 2016 | 6 | 0 | |
| Leptophlebia vespertina | Hampshire, UK | 2016 | 1 | 0 | |
| Paraleptophlebia submarginata | Stirling, Scotland, UK | 2017 | 3 | 0 | |
| Rhithrogena semicolorata | Stirling, Scotland, UK | 2017 | 3 | 0 | |
| Trichoptera | Hydropsyche sp. | Stirling, Scotland, UK | 2017 | 3 | 0 |
| Polycentropus flavomaculatus | Cheshire, UK | 2017 | 3 | 0 | |
| Rhyacophila dorsalis | Stirling, Scotland, UK | 2017 | 3 | 2 | |
| Plecoptera | Amphinemura sulcicollis | Stirling, Scotland, UK | 2017 | 3 | 0 |
| Dinocras cephalotes | Stirling, Scotland, UK | 2017 | 3 | 0 | |
| Isoperla grammatica | Stirling, Scotland, UK | 2017 | 3 | 0 | |
| Perla bipunctata | Stirling, Scotland, UK | 2017 | 3 | 0 | |
| Hemiptera | Corixa punctata | Cheshire, UK | 2016 | 1 | 0 |
| Gerris sp. | Montferrier sur Lez, France | 2006 | 12 | 0 | |
| Gerris thoracicus | Cheshire, UK | 2016 | 1 | 0 | |
| Hydrometra stagnorum | Montferrier sur Lez, France | 2006 | 20 | 0 | |
| Nepa cinerea | Montferrier sur Lez, France | 2006 | 3 | 0 | |
| Notonecta glauca | Cheshire, UK | 2016 | 2 | 0 | |
| Plea minutissima | Notre Dame de Londres, France | 2006 | 8 | 0 | |
| Sigara lateralis | Notre Dame de Londres, France | 2006 | 6 | 0 | |
| Sigara striata | Cheshire, UK | 2006 | 2 | 1 | |
| Diptera | Aedes sp. | Cheshire, UK | 2017 | 8 | 0 |
| Aedes albopictus | Roma, Italy | 2005 | 20 | 0 | |
| Anopheles plumbeus | Chester Zoo, UK | 2018 | 2 | 2 | |
| Chironomidae sp. | Cheshire, UK | 2016 | 4 | 1 | |
| Chironomus acidophilus | Cheshire, UK | 2017 | 1 | 0 | |
| Chironomus plumosus | Notre Dame de Londres, France | 2006 | 20 | 0 | |
| Chironomus sp. | Cheshire, UK | 2016 | 4 | 0 | |
| Culex pipiens (ssp. quinquefasciatus) | Puerto Viejo de Talamanca, Costa Rica | 2006 | 20 | 0 | |
| Culex pipiens | St Nazaire de Pézan, France | 2006 | 20 | 0 | |
| Eristalinus sp. | Cheshire, UK | 2016 | 3 | 0 | |
| Eristalis tenax | Montpellier (grotte du zoo), France | 2002 | 7 | 0 | |
| Glyptotendipes sp. | Cheshire, UK | 2016 | 1 | 1 | |
| Hilarainterstincta | Cheshire, UK | 2017 | 3 | 1 | |
| Simulium aureum | Hampshire, UK | 2017 | 1 | 1 | |
| Simulium ornatum | N/A | 2003 | 12 | 0 | |
| Tipula sp. | UK | 2006 | 10 | 0 | |
| Tipula oleracea | UK | 2006 | 13 | 0 | |
| Zavrelimyia sp. | Northumberland, UK | 2017 | 1 | 1 | |
| Coleoptera | Agabus bipustulatus | Cheshire, UK | 2017 | 3 | 0 |
| Guignotus pusillus | Notre Dame de Londres, France | 2006 | 12 | 0 | |
| Unknown sp.1 | Cheshire, UK | 2017 | 2 | 0 | |
| Unknown sp.2 | Cheshire, UK | 2017 | 3 | 0 | |
| Acarina | Unknown sp. | Cheshire, UK | 2017 | 3 | 0 |
| Isopoda | Asellus aquaticus | Cheshire, UK | 2016 | 3 | 0 |
| Amphipoda | Gammarus pulex | Stirling, Scotland, UK | 2017 | 3 | 0 |
| Crangonyx pseudogracilis | Cheshire, UK | 2016 | 6 | 0 | |
| Gastropoda | Radix balthica | Cheshire, UK | 2016 | 3 | 0 |
| Planorbis sp. | Cheshire, UK | 2016 | 3 | 0 | |
| Galba truncatula | Cheshire, UK | 2017 | 20 | 3 | |
| Hirudinea | Erpobdella octoculata | Cheshire, UK | 2016 | 2 | 0 |
| Hemiclepsis marginata | Cheshire, UK | 2017 | 1 | 0 | |
| Tricladida | Unknown sp. | Cheshire, UK | 2016 | 1 | 0 |
A species was deemed positive through PCR and designated to Rickettsia group after Sanger sequencing and phylogenetic placement. All strains belong to the Torix group. Bold entries indicate Rickettsia-positive hosts identified in this study.