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. 2021 Feb 11;10:e61408. doi: 10.7554/eLife.61408

Author response image 1. Preservation of regions demarcated by ISH markers.

Author response image 1.

(A) The Wnt3a ISH signal (dashed circle) shown in Thompson CL, et al. (2014, Figure 2; top row) is expressed selectively within the cortical hem in the original labels of the E13.5 atlas (lower middle row, orange structure) and remains contained in this region in the 3D reconstructed atlas (bottom row). (B) The Hoxa2 ISH signal demarcates the border between the pontine hindbrain and prepontine hindbrain in the E15.5 atlas (top row). This boundary remains well-demarcated before (lower middle row) and after (bottom row) 3D reconstruction.