Table 2.

Prospective Cost Comparison between Enzymatic and Chemical Methods of Oligonucleotide Synthesis^a

method	cost (USD) per dNTPPr incorporationb	cost (USD) of TdT	cost (USD) of dNTPPr	total cost (USD) of TdT + dNTPPrc
TiEOS with TdTd	1.30 × 10−7	1.36 × 105	1.30 × 10−1	1.36 × 105
TiEOS without TdTe	1.30 × 10−7	1.36 × 102	1.30 × 10−1	1.36 × 102
phosphoramidite	2.70 × 10−3	–	–	2.70 × 103

^aValues in this table represent total costs for 1 fm-scale production of 1000 samples, at 1000 nt/strand, where the rate of nucleotide incorporation is estimated to be 250 fm s⁻¹ (unit of TdT)⁻¹.⁶¹

^bIncludes cost for nucleoside phosphoramidite additions.

^cTotal cost for phosphoramidite method also included.

^dFresh TdT .

^erecycled TdT (attached to solid substrate). All costs reflect current market pricing.