Figure 2.

Compatibility test of the single reactions in the cascade: (A) Single reaction steps of the cascade with substrates, co-substrates, products, and effector. (B) Relative activity of the enzymes at 40 °C in a pH-range from 7.0 to 9.0. 100% relative activity corresponds to 324 U mg⁻¹ (APT), 27 U mg⁻¹ (PPK), and 0.52 U mg⁻¹ (RNR), respectively. PPK activity was determined for the conversion of AMP to ADP. (C) Relative activity of the enzymes at a pH-value of 8.0 in a temperature range from 20 °C to 50 °C. 100% relative activity corresponds to 586 U mg⁻¹ (APT), 55 U mg⁻¹ (PPK), and 0.78 U mg⁻¹ (RNR), respectively. PPK activity was determined for the conversion of AMP to ADP. (D) Relative activity of the enzymes at 40 °C and a pH-value of 8.0 in the presence of substrates and effector from the other reaction steps. 100% relative activity corresponds to 391 U mg⁻¹ (APT), 31 U mg⁻¹ (PPK), and 0.44 U mg⁻¹ (RNR), respectively. PPK activity was determined for the conversion of AMP to ADP.