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. 2021 Feb 25;11(3):346. doi: 10.3390/biom11030346

Figure A3.

Figure A3

LC-MS identification of 2Cl-adenosine (deoxy)ribonucleotides: (A) LC and full-MS spectra of the reaction of APT only with 2Cl-adenine as substrate. Identified peaks: 1: 1.57 min, 2Cl-AMP; 2: 5.78 min, dGTP. (B) LC and full-MS spectra of the reaction of APT and PPK with 2Cl-adenine as substrate. Identified peaks: 1: 3.63 min, 2Cl-ADP; 2: 5.02 min, 2Cl-ATP. (C) LC and full-MS spectra of the adenylate kinase test reaction of APT and PPK with 2Cl-adenine as substrate and 2Cl-dATP as phosphate donor. Identified peaks: 1: 1.58 min, 2Cl-AMP; 2: 3.02 min, 2Cl-dADP. 3.51 min, 2Cl-ADP; 4.64 min. 2CL-dATP. (D) LC and full-MS spectra of the Reaction of the full cascade with 2Cl-adenine as substrate. Identified peaks as 1: 1.48 min, 2Cl-dAMP; 2: 3.17 min, 2Cl-dADP; 3: 4.65 min, 2Cl-dATP.