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Acta Veterinaria Scandinavica logoLink to Acta Veterinaria Scandinavica
. 2000 Dec 1;41(4):351–361. doi: 10.1186/BF03549626

The Effect of Birdsfoot Trefoil (Lotus corniculatus) and White Clover (Trifolium repens) in Mixed Pasture Swards on Incoming and Established Nematode Infections in Young Lambs

Effekt av betesvall innehållande käringtand (Lotus corniculatus L.) eller vitklöver (Trifolium repens L.) på inkommande och etablerade parasitinfektioner hos unga lamm

G Bernes 1, P J Waller 2,, D Christensson 2
PMCID: PMC7996412  PMID: 11234969

Abstract

Lotus corniculatus L., a leguminous plant containing condensed tannins, is now being evaluated in Sweden as a component of mixed pastures. This is because of its high nutritive value, palatability, modest requirement for water, calcium and phosphorous and particularly because it has the ability to survive harsh weather conditions that typify northern Scandinavia. This trial was undertaken to assess the possible parasitological benefits of using L. corniculatus when fed as a minor component in a mixed pasture sward to young sheep. This was compared with similar sheep that were fed pasture with a comparable legume content consisting of Trifolium repens L. Separate groups of lambs, maintained in pens and provided with fresh pasture cuts containing either L. corniculatus or T. repens each day, were trickle-dosed with infective larvae of mixed parasite species. For each pasture type, groups of 6 lambs had either established worm burdens only, received incoming infections only, or had the combination of both adult and incoming infections. Worm burdens were monitored by serial faecal egg counts and at the termination of the study, all animals were slaughtered for worm recovery, identification and enumeration. The results showed that there were no differential effects between L. corniculatus and T. repens on the different stages of development for a range of nematode parasite species recovered from these lambs. Several reasons may account for this: such as the low level of condensed tannins in the L. corniculatus variety tested, the minor proportion of this plant in the diet and/or the variety of L. corniculatus used in this trial which do not provide any benefits to controlling parasites of sheep.

Keywords: condensed tannins, nematode parasites, sheep

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References

  1. Aerts RJ, Barry TN, McNabb WC. Polyophenols and agriculture: beneficial effects of proanthocyanidins in forages. Agriculture, Ecosystems and Environment. 1999;75:1–12. doi: 10.1016/S0167-8809(99)00062-6. [DOI] [Google Scholar]
  2. Abbott EM, Parkins JJ, Holmes PH. Influence of dietry protein on parasite establishment and pathogenesis in Finn Dorset and Scottish Blackface lambs given a single moderate infection of Haemonchus contortus. Res. Vet. Sci. 1985;38:6–13. doi: 10.1016/S0034-5288(18)31840-X. [DOI] [PubMed] [Google Scholar]
  3. Barger IA, Le Jambre LF, Georgi JR, Davies HI. Regulation of Haemonchus contortus populations in sheep exposed to continuous infection. Int. J. Parasitol. 1985;15:529–533. doi: 10.1016/0020-7519(85)90049-9. [DOI] [PubMed] [Google Scholar]
  4. Beuselinck PR, Grant WF. Birdsfoot trefoil. In: Barnes RF, Miller DA, Nelson CJ, editors. Forages Volume 1: An Introduction to Grassland Agriculture. Ames, Iowa, USA: Iowa State University Press; 1995. pp. 237–248. [Google Scholar]
  5. Brown MD, Poppi DP, Sykes AR. The effect of post-ruminal infusion of protein or energy on the pathophysiology of Trichostrongylus colubriformis infection and body compostion in lambs. Aust. J. Agric. Res. 1991;42:253–267. doi: 10.1071/AR9910253. [DOI] [Google Scholar]
  6. Dobson RJ, Waller PJ, Donald AD. Population dynamics of Trichostrongylus colubriformis in sheep; the effect of infection rate on the establishment of infective larvae and parasite fecundity. Int. J. Parasitol. 1990;20:347–352. doi: 10.1016/0020-7519(90)90150-L. [DOI] [PubMed] [Google Scholar]
  7. Dobson RJ, Waller PJ, Donald AD. Population dynamics of Trichostrongylus colubriformis in sheep: the effect of host age on the establishment of infective larvae. Int. J. Parasitol. 1990;20:353–357. doi: 10.1016/0020-7519(90)90151-C. [DOI] [PubMed] [Google Scholar]
  8. Dobson RJ, Waller PJ, Donald AD. Population dynamics of Trichostrongylus colubriformis in sheep; the effect of infection rate on loss of adult parasites. Int. J. Parasitol. 1990;20:359–363. doi: 10.1016/0020-7519(90)90152-D. [DOI] [PubMed] [Google Scholar]
  9. Dobson RJ, Donald AD, Barnes EH, Waller PJ. Population dynamics of Trichostrongylus colubriformis in sheep; model to predict the worm population over time as a function of infection rate and host age. Int. J. Parasitol. 1990;20:365–373. doi: 10.1016/0020-7519(90)90153-E. [DOI] [PubMed] [Google Scholar]
  10. Donald AD, Morley FHW, Waller PJ, Axelsen A, Donnelly JR. Availability to grazing sheep of gastrointestinal nematode infection arising from summer contamination of pastures. Aust. J. Agric.Res. 1978;29:189–204. doi: 10.1071/AR9780189. [DOI] [Google Scholar]
  11. Hagerman AE. Radial diffusion method for determining tannin in plant extracts. J. Chem. Ecol. 1987;13:437–449. doi: 10.1007/BF01880091. [DOI] [PubMed] [Google Scholar]
  12. Hedqvist H: Kondenserade tanniner i käringtand (Lotus corniculatus L.) - kvantifiering, karaktärisering samt in vitro studier av deras effekt på proteinnedbrytning i vommen (Condensed tannins in birdsfoot trefoil (Lotus corniculatus L.) - quantification, characterization and in vitro studies of their effect on protein degradation in the rumen). 1999. Examensarbete 116, inst. för husdjurens utfodring och vård, SLU.
  13. Hedqvist H, Mueller-Harvey I, Reed JD, Krueger CG, Murphy M: The tannins and in vitro digestibility of seven Lotus corniculatus varieties. Anim. Feed. Sci. Techn. 2000. (in press).
  14. Hintze J: NCSS 2000. Manual. 1998. Kaysville, Utah.
  15. Hong C, Michel JF, Lancaster MB. Observations on the dynamics of worm burdens in lambs infected daily with Ostertagia circumcincta. Int. J. Parasit. 1987;17:951–956. doi: 10.1016/0020-7519(87)90014-2. [DOI] [PubMed] [Google Scholar]
  16. Jones WT, Mangan JL. Complexes of condensed tannin of sanfoin (Onobrychus vicifolia Scop) with fraction 1 leaf protein and with submaxillary mucoprotein, and their reversal by polyethylene glycol and pH. J. Sci. Food. Agric. 1977;28:126–136. doi: 10.1002/jsfa.2740280204. [DOI] [Google Scholar]
  17. Kahn LP, Diaz-Hernandez A: Tannins with anthelmintic properties. International Workshop on Tannins in Livestock and Human Nutrition. Waite Campus, University of Adelaide. 1999. ACIAR Proceedings No 92, 1999, 130–139.
  18. Kelman WM, Tanner GJ. Foliar condensed tannin levels in lotus species growing on limed and unlimed soils in South-Eastern Australia. Proc. N.Z. Grassland Assoc. 1990;52:51–54. doi: 10.33584/jnzg.1990.52.1934. [DOI] [Google Scholar]
  19. Lowther WL, Manley TR, Barry TN. Condensed tannin concentrations in Lotus corniculatus and Lotus pedunculatus cultivars grown under low soil fertility conditions. N.Z. J. Agric. Res. 1987;30:23–25. doi: 10.1080/00288233.1987.10430473. [DOI] [Google Scholar]
  20. Molan AL, Waghorn GC, McNabb WC. Condensed tannins and gastro-intestinal parasites in sheep. Proceedings of the New Zealand Grassland Association. 1999;61:57–61. doi: 10.33584/jnzg.1999.61.2363. [DOI] [Google Scholar]
  21. Niezen JH, Waghorn TS, Raufaut K, Robertson HA, McFarlane RG. Lamb weight gain and faecal egg count when grazing on of seven herbages and dosed with larvae for six weeks. Proc. NZ Soc. Anim. Prod. 1994;54:15–18. [Google Scholar]
  22. Niezen JH, Waghorn TS, Charleston WAG, Waghorn GC. Growth and gastrointestinal nematode parasitism in lambs grazing either lucerne (Medicago sativa) or sulla (Hedysarum coronarium) which contains condensed tannins. J. Agric. Sci. Camb. 1995;125:281–289. doi: 10.1017/S0021859600084422. [DOI] [Google Scholar]
  23. Niezen JH, Waghorn GC, Charleston WAG. Establishment and fecundity of Ostertagia circumcincta and Trichostrongylus colubriformis in lambs fed lotus (Lotus pedunculatus) or perennial ryegrass (Lolium perenne). Vet. Parasitol. 1998;78:13–21. doi: 10.1016/s0304-4017(98)00121-6. [DOI] [PubMed] [Google Scholar]
  24. Nilsdotter-Linde N: Birdsfoot trefoil grown in mixtures with grasses in a temperate climate. In Organic agriculture the credible solution for the XXIst century. (D. Fougelman & W. Lockeretz -editors). Proceedings of the 12th international IFOAM scientific conference. November 15th–19th 1998. Mar del Plata, Argentina, 1999, pp.171–175.
  25. Nilsdotter-Linde N, Tuvesson M: Samodlingskomponenter till käringtand (Lotus corniculatus L.) (Companion plants to birdsfoot trefoil (Lotus corniculatus L.). SLU Centrum för uthålligt lantbruk. Konferens Ekologiskt lantbruk, Alnarp, 8–10 november 1999. Sammanfattningar av föredrag och postrar, 173–174.
  26. Nilsdotter-Linde N, Bernes G, Christensson D, Jansson J, Murphy M, Tuvesson M. Tar käringtanden klivet ut på åkern? – populationsekologi, proteinutnyttjande, parasitpåverkan (Does birdsfoot trefoil take the step out into the field? – population ecology, protein utilization, effect on parasite. SLU. CUL. Ekologiskt lantbruk 10–13 mars 1998. Konferensrapport. Ekologiskt lantbruk. 1999;28:142–143. [Google Scholar]
  27. Porter LW, Hrstich LN, Chan BG. The conversion of procyanidins and prodelphinidins to cyanidin and delphinidin. Phytochemistry. 1986;25:223–230. doi: 10.1016/S0031-9422(00)94533-3. [DOI] [Google Scholar]
  28. Robertson HA, Niezen JH, Waghorn GC, Charleston WAG, Jinlong M. The effect of six herbages on liveweight gain, wool growth, and faecal egg count of parasitised ewe lambs. Proc. NZ. Soc. Anim. Prod. 1995;55:199–201. [Google Scholar]
  29. SAS . SAS System for Windows, version 6.12. User’s guide; Statistics. Cary, NC: SAS Institute Inc.; 1996. [Google Scholar]
  30. Tanner GJ, Moate PJ, Davis LH, Laby RH, Yuguang L, Larkin PJ. Proanthocyanidins (condensed tannin) destabilise plant protein foams in a dose dependent manner. Austr. J. Agric. Res. 1995;46:1101–1109. doi: 10.1071/AR9951101. [DOI] [Google Scholar]
  31. Van Houtert MFJ, Barger IA, Steel JW, Windon RG, Emery DL. Effects of dietary protein intake on responses of young sheep to infection with Trichostrongylus colubriformis. Vet. Parasitol. 1995;56:163–180. doi: 10.1016/0304-4017(94)00668-3. [DOI] [PubMed] [Google Scholar]
  32. Waghorn GC, Ulyatt MJ, John A, Fisher MT. The effect of condensed tannins on the site of digestion of amino acids and other nutrients in sheep fed on Lotus corniculatus L. British J. Nut. 1987;57:115–126. doi: 10.1079/BJN19870015. [DOI] [PubMed] [Google Scholar]
  33. Waghorn GC, Shelton ID, McNabb WC, McCutcheon SN. Effects of condensed tannins in Lotus pedunculatus on nutiritive value for sheep. 2. Nitrogenous aspects. J. Agric. Sci. Camb. 1994;123:109–119. doi: 10.1017/S0021859600067836. [DOI] [Google Scholar]
  34. Waghorn GC, Shelton ID. Effect of condensed tannins in Lotus corniculatus on the nutritive value of pasture for sheep. J. Agric. Sci. 1997;128:365–372. doi: 10.1017/S0021859697004218. [DOI] [Google Scholar]

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